BMRB Entry 53572
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53572
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Levasseur, Mikail; Terasaka, Naohiro; Steinauer, Angela; Tetter, Stephan; Pfister, Sara; Meier, Beat; Hilvert, Donald. "An engineered closed-shell, two-component, 480-subunit nucleocapsid" Proc. Natl. Acad. Sci. U.S.A. ., .-..
Assembly members:
entity_1, polymer, 113 residues, Formula weight is not available
entity_2, polymer, 86 residues, Formula weight is not available
Natural source: Common Name: Aquifex aeolicus Taxonomy ID: 63363 Superkingdom: Bacteria Kingdom: not available Genus/species: Aquifex aeolicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 422 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 204 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | spNC-4 N-terminal fragment | 1 |
| 2 | spNC-4 C-terminal fragment | 2 |
Entities:
Entity 1, spNC-4 N-terminal fragment 113 residues - Formula weight is not available
| 1 | MET | GLY | ASN | ALA | ARG | THR | ARG | ARG | ARG | GLU | ||||
| 2 | ARG | ARG | ALA | GLU | LYS | GLN | ALA | GLN | TRP | LYS | ||||
| 3 | ALA | ALA | ASN | ALA | GLY | ALA | GLY | ALA | GLY | ALA | ||||
| 4 | MET | ALA | THR | PRO | HIS | PHE | ASP | TYR | ASN | ALA | ||||
| 5 | SER | VAL | VAL | SER | LYS | GLY | LEU | ALA | ASN | LEU | ||||
| 6 | SER | LEU | GLU | LEU | ARG | LYS | PRO | VAL | SER | PHE | ||||
| 7 | ASP | ILE | ILE | THR | ALA | ASP | THR | LEU | GLU | GLN | ||||
| 8 | ALA | ILE | GLU | ARG | ALA | GLY | THR | LYS | HIS | GLY | ||||
| 9 | ASN | LYS | GLY | TRP | GLU | ALA | ALA | LEU | SER | ALA | ||||
| 10 | ILE | GLU | MET | ALA | ASN | LEU | TYR | LYS | SER | LEU | ||||
| 11 | ARG | GLY | THR | GLU | HIS | HIS | HIS | HIS | LEU | HIS | ||||
| 12 | GLY | SER | SER |
Entity 2, spNC-4 C-terminal fragment 86 residues - Formula weight is not available
| 1 | MET | GLY | ILE | GLU | ILE | TYR | GLU | GLY | LYS | LEU | ||||
| 2 | THR | ALA | GLU | GLY | LEU | ARG | PHE | GLY | ILE | VAL | ||||
| 3 | ALA | SER | ARG | PHE | ASN | HIS | THR | LEU | VAL | ASP | ||||
| 4 | ARG | LEU | VAL | GLU | GLY | ALA | ILE | ASP | CYS | ILE | ||||
| 5 | VAL | ARG | HIS | GLY | GLY | ARG | GLY | GLU | ASP | ILE | ||||
| 6 | THR | LEU | VAL | ARG | VAL | PRO | GLY | ALA | TRP | GLU | ||||
| 7 | ILE | PRO | VAL | ALA | ALA | ASP | GLU | LEU | ALA | ARG | ||||
| 8 | LYS | GLU | ASP | ILE | ASP | ALA | VAL | ILE | ALA | PHE | ||||
| 9 | GLY | ASP | LEU | ILE | ARG | GLY |
Samples:
sample_1: spNC-4 480-subunit T=4 icosahedral capsid, [U-13C; U-15N], na na; H2O na na; DSS na na; sodium azide na na
sample_conditions_1: pH: 7.4; temperature: 277 K
sample_conditions_2: pH: 7.4; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-13C DARR | sample_1 | isotropic | sample_conditions_1 |
| 2D NCA | sample_1 | isotropic | sample_conditions_1 |
| 2D NCO | sample_1 | isotropic | sample_conditions_1 |
| 2D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 2D hNH | sample_1 | isotropic | sample_conditions_2 |
| 2D hCH | sample_1 | isotropic | sample_conditions_2 |
| 3D hCANH | sample_1 | isotropic | sample_conditions_2 |
Software:
TOPSPIN v3.5 - Spectral processing
CcpNMR v2 - Spectral analysis and resonance assignment
NMR spectrometers:
- Bruker AVANCE III 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks