Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53571

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Aquifex aeolicus lumazine synthase-derived nucleocapsid variant NC-4
Published: 2026-05-15

Title:

Aquifex aeolicus lumazine synthase-derived nucleocapsid variant NC-4

Deposition date:

2026-02-12

Original release date:

2026-05-15

Authors:

Levasseur, Mikail; Terasaka, Naohiro; Steinauer, Angela; Tetter, Stephan; Pfister, Sara; Meier, Beat; Hilvert, Donald

Citation:

Citation: Levasseur, Mikail; Terasaka, Naohiro; Steinauer, Angela; Tetter, Stephan; Pfister, Sara; Meier, Beat; Hilvert, Donald. "An engineered closed-shell, two-component, 480-subunit nucleocapsid" Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 197 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Aquifex aeolicus Taxonomy ID: 63363 Superkingdom: Bacteria Kingdom: not available Genus/species: Aquifex aeolicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGNARTRRRERRAEKQAQWK AANAGAGAGAMATPHFDYNA SVVSKGLANLSLELRKPVSF DIITADTLEQAIERAGTKHG NKGWEAALSAIEMANLYKSL RGTEHHHHLHGSSIEIYEGK LTAEGLRFGIVASRFNHTLV DRLVEGAIDCIVRHGGRGED ITLVRVPGAWEIPVAADELA RKEDIDAVIAFGDLIRG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	389
15N chemical shifts	103
1H chemical shifts	71

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NC-4 monomer	1

Entities:

Entity 1, NC-4 monomer 197 residues - Formula weight is not available

1

MET

GLY

ASN

ALA

ARG

THR

ARG

GLU

2

ARG

ALA

GLU

LYS

GLN

ALA

GLN

TRP

LYS

3

ALA

ASN

ALA

GLY

ALA

GLY

ALA

GLY

ALA

4

MET

ALA

THR

PRO

HIS

PHE

ASP

TYR

ASN

ALA

5

SER

VAL

SER

LYS

GLY

LEU

ALA

ASN

LEU

6

SER

LEU

GLU

LEU

ARG

LYS

PRO

VAL

SER

PHE

7

ASP

ILE

THR

ALA

ASP

THR

LEU

GLU

GLN

8

ALA

ILE

GLU

ARG

ALA

GLY

THR

LYS

HIS

GLY

9

ASN

LYS

GLY

TRP

GLU

ALA

LEU

SER

ALA

10

ILE

GLU

MET

ALA

ASN

LEU

TYR

LYS

SER

LEU

11

ARG

GLY

THR

GLU

HIS

LEU

HIS

12

GLY

SER

ILE

GLU

ILE

TYR

GLU

GLY

LYS

13

LEU

THR

ALA

GLU

GLY

LEU

ARG

PHE

GLY

ILE

14

VAL

ALA

SER

ARG

PHE

ASN

HIS

THR

LEU

VAL

15

ASP

ARG

LEU

VAL

GLU

GLY

ALA

ILE

ASP

CYS

16

ILE

VAL

ARG

HIS

GLY

ARG

GLY

GLU

ASP

17

ILE

THR

LEU

VAL

ARG

VAL

PRO

GLY

ALA

TRP

18

GLU

ILE

PRO

VAL

ALA

ASP

GLU

LEU

ALA

19

ARG

LYS

GLU

ASP

ILE

ASP

ALA

VAL

ILE

ALA

20

PHE

GLY

ASP

LEU

ILE

ARG

GLY

Samples:

sample_1: Aquifex aeolicus lumazine synthase-derived nucleocapsid variant NC-4, [U-13C; U-15N], na na; H2O na na; DSS na na; sodium azide na na

sample_conditions_1: pH: 7.4; temperature: 4 K

sample_conditions_2: pH: 7.4; temperature: 20 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C DARR	sample_1	isotropic	sample_conditions_1
2D NCA	sample_1	isotropic	sample_conditions_1
2D NCO	sample_1	isotropic	sample_conditions_1
2D NCACX	sample_1	isotropic	sample_conditions_1
2D hNH	sample_1	isotropic	sample_conditions_2
2D hCH	sample_1	isotropic	sample_conditions_2
3D hCANH	sample_1	isotropic	sample_conditions_2

Software:

TOPSPIN v3.5 - Spectral processing

CcpNMR v2 - Spectral analysis and resonance assignment

NMR spectrometers:

Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 53571