Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53501

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Chemical Shift assignments of Human MLLT3 YEATS domain
Published: 2026-02-10

Title:

Chemical Shift assignments of Human MLLT3 YEATS domain

Deposition date:

2026-01-12

Original release date:

2026-02-10

Authors:

Kabra, Ashish; Boulton, Adam; Leach, Benjamin; Bushweller, John

Citation:

Citation: Boulton, Adam; Kabra, Ashish; Achille, Nicholas; Adelman, Emmalee; Anastasakis, Dimitrios; Beckedorff, Felipe; Cingaram, Pradeepkumar; Zhang, Ying; Leach, Benjamin; Shiekhattar, Ramin; Yokoyama, Akihiko; Hafner, Markus; Figueroa, Maria; Zeleznik-Le, Nancy; Bushweller, John. "The MLLT3 YEATS domain is a dual reader of histone marks (H3K9/18/27ac/cr) and ncRNA (7SK), linking epigenetic and RNA signaling to regulate hematopoiesis" Cell Reports ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MASSAAVQVKLELGHRAQVR KKPTVEGFTHDWMVFVRGPE HSNIQHFVEKVVFHLHESFP RPKRVCKDPPYKVEESGYAG FILPIEVYFKNKEEPRKVRF DYDLFLHLEGHPPVNHLRCE KLTFNNPTEDFRRKLLKAGG LEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	109
1H chemical shifts	109

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MLLT3 YEATS domain	1

Entities:

Entity 1, MLLT3 YEATS domain 148 residues - Formula weight is not available

1

MET

ALA

SER

ALA

VAL

GLN

VAL

LYS

2

LEU

GLU

LEU

GLY

HIS

ARG

ALA

GLN

VAL

ARG

3

LYS

PRO

THR

VAL

GLU

GLY

PHE

THR

HIS

4

ASP

TRP

MET

VAL

PHE

VAL

ARG

GLY

PRO

GLU

5

HIS

SER

ASN

ILE

GLN

HIS

PHE

VAL

GLU

LYS

6

VAL

PHE

HIS

LEU

HIS

GLU

SER

PHE

PRO

7

ARG

PRO

LYS

ARG

VAL

CYS

LYS

ASP

PRO

8

TYR

LYS

VAL

GLU

SER

GLY

TYR

ALA

GLY

9

PHE

ILE

LEU

PRO

ILE

GLU

VAL

TYR

PHE

LYS

10

ASN

LYS

GLU

PRO

ARG

LYS

VAL

ARG

PHE

11

ASP

TYR

ASP

LEU

PHE

LEU

HIS

LEU

GLU

GLY

12

HIS

PRO

VAL

ASN

HIS

LEU

ARG

CYS

GLU

13

LYS

LEU

THR

PHE

ASN

PRO

THR

GLU

ASP

14

PHE

ARG

LYS

LEU

LYS

ALA

GLY

15

LEU

GLU

HIS

Samples:

sample_1: MLLT3 YEATS domain, [U-99% 15N], 250 uM; potassium phosphate 50 mM; potassium chloride 50 mM; DTT 2 mM; DSS 1 mM

sample_2: MLLT3 YEATS domain, [U-99% 13C; U-99% 15N], 350 uM; potassium phosphate 50 mM; potassium chloride 50 mM; DTT 2 mM; DSS 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCACO	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe - processing

CcpNMR - chemical shift assignment

NMRFAM-SPARKY - data analysis

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 53501