BMRB Entry 53477

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53477

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Title:
Resonance assignments of human tropoelastin (hTE)
Deposition date:
2025-12-09
Original release date:
2026-02-06
Authors:
Reichheld, Sean; Sharpe, Simon
Citation:

Citation: Reichheld, Sean; Muiznieks, Lisa; Liu, Zi Hao; Payliss, Brandon; Keeley, Fred; Sharpe, Simon. "A free energy landscape screen reveals the disordered conformational ensemble of tropoelastin"  .

Assembly members:

Assembly members:
entity_1, polymer, 698 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet32b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGVPGAIPGGVPGGVFYPGA GLGALGGGALGPGGKPLKPV PGGLAGAGLGAGLGAFPAVT FPGALVPGGVADAAAAYKAA KAGAGLGGVPGVGGLGVSAG AVVPQPGAGVKPGKVPGVGL PGVYPGGVLPGARFPGVGVL PGVPTGAGVKPKAPGVGGAF AGIPGVGPFGGPQPGVPLGY PIKAPKLPGGYGLPYTTGKL PYGYGPGGVAGAAGKAGYPT GTGVGPQAAAAAAAKAAAKF GAGAAGVLPGVGGAGVPGVP GAIPGIGGIAGVGTPAAAAA AAAAAKAAKYGAAAGLVPGG PGFGPGVVGVPGAGVPGVGV PGAGIPVVPGAGIPGAAVPG VVSPEAAAKAAAKAAKYGAR PGVGVGGIPTYGVGAGGFPG FGVGVGGIPGVAGVPGVGGV PGVGGVPGVGISPEAQAAAA AKAAKYGVGTPAAAAAKAAA KAAQFGLVPGVGVAPGVGVA PGVGVAPGVGLAPGVGVAPG VGVAPGVGVAPGIGPGGVAA AAKSAAKVAAKAQLRAAAGL GAGIPGLGVGVGVPGLGVGA GVPGLGVGAGVPGFGAVPGA LAAAKAAKYGAAVPGVLGGL GALGGVGIPGGVVGAGPAAA AAAAKAAAKAAQFGLVGAAG LGGLGVGGLGVPGVGGLGGI PPAAAAKAAKYGAAGLGGVL GGAGQFPLGGVAARPGFGLS PIFPGGACLGKACGRKRK

Data sets:
Data typeCount
13C chemical shifts1052
15N chemical shifts516
1H chemical shifts492

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hTE1

Entities:

Entity 1, hTE 698 residues - Formula weight is not available

1   GLYGLYVALPROGLYALAILEPROGLYGLY
2   VALPROGLYGLYVALPHETYRPROGLYALA
3   GLYLEUGLYALALEUGLYGLYGLYALALEU
4   GLYPROGLYGLYLYSPROLEULYSPROVAL
5   PROGLYGLYLEUALAGLYALAGLYLEUGLY
6   ALAGLYLEUGLYALAPHEPROALAVALTHR
7   PHEPROGLYALALEUVALPROGLYGLYVAL
8   ALAASPALAALAALAALATYRLYSALAALA
9   LYSALAGLYALAGLYLEUGLYGLYVALPRO
10   GLYVALGLYGLYLEUGLYVALSERALAGLY
11   ALAVALVALPROGLNPROGLYALAGLYVAL
12   LYSPROGLYLYSVALPROGLYVALGLYLEU
13   PROGLYVALTYRPROGLYGLYVALLEUPRO
14   GLYALAARGPHEPROGLYVALGLYVALLEU
15   PROGLYVALPROTHRGLYALAGLYVALLYS
16   PROLYSALAPROGLYVALGLYGLYALAPHE
17   ALAGLYILEPROGLYVALGLYPROPHEGLY
18   GLYPROGLNPROGLYVALPROLEUGLYTYR
19   PROILELYSALAPROLYSLEUPROGLYGLY
20   TYRGLYLEUPROTYRTHRTHRGLYLYSLEU
21   PROTYRGLYTYRGLYPROGLYGLYVALALA
22   GLYALAALAGLYLYSALAGLYTYRPROTHR
23   GLYTHRGLYVALGLYPROGLNALAALAALA
24   ALAALAALAALALYSALAALAALALYSPHE
25   GLYALAGLYALAALAGLYVALLEUPROGLY
26   VALGLYGLYALAGLYVALPROGLYVALPRO
27   GLYALAILEPROGLYILEGLYGLYILEALA
28   GLYVALGLYTHRPROALAALAALAALAALA
29   ALAALAALAALAALALYSALAALALYSTYR
30   GLYALAALAALAGLYLEUVALPROGLYGLY
31   PROGLYPHEGLYPROGLYVALVALGLYVAL
32   PROGLYALAGLYVALPROGLYVALGLYVAL
33   PROGLYALAGLYILEPROVALVALPROGLY
34   ALAGLYILEPROGLYALAALAVALPROGLY
35   VALVALSERPROGLUALAALAALALYSALA
36   ALAALALYSALAALALYSTYRGLYALAARG
37   PROGLYVALGLYVALGLYGLYILEPROTHR
38   TYRGLYVALGLYALAGLYGLYPHEPROGLY
39   PHEGLYVALGLYVALGLYGLYILEPROGLY
40   VALALAGLYVALPROGLYVALGLYGLYVAL
41   PROGLYVALGLYGLYVALPROGLYVALGLY
42   ILESERPROGLUALAGLNALAALAALAALA
43   ALALYSALAALALYSTYRGLYVALGLYTHR
44   PROALAALAALAALAALALYSALAALAALA
45   LYSALAALAGLNPHEGLYLEUVALPROGLY
46   VALGLYVALALAPROGLYVALGLYVALALA
47   PROGLYVALGLYVALALAPROGLYVALGLY
48   LEUALAPROGLYVALGLYVALALAPROGLY
49   VALGLYVALALAPROGLYVALGLYVALALA
50   PROGLYILEGLYPROGLYGLYVALALAALA
51   ALAALALYSSERALAALALYSVALALAALA
52   LYSALAGLNLEUARGALAALAALAGLYLEU
53   GLYALAGLYILEPROGLYLEUGLYVALGLY
54   VALGLYVALPROGLYLEUGLYVALGLYALA
55   GLYVALPROGLYLEUGLYVALGLYALAGLY
56   VALPROGLYPHEGLYALAVALPROGLYALA
57   LEUALAALAALALYSALAALALYSTYRGLY
58   ALAALAVALPROGLYVALLEUGLYGLYLEU
59   GLYALALEUGLYGLYVALGLYILEPROGLY
60   GLYVALVALGLYALAGLYPROALAALAALA
61   ALAALAALAALALYSALAALAALALYSALA
62   ALAGLNPHEGLYLEUVALGLYALAALAGLY
63   LEUGLYGLYLEUGLYVALGLYGLYLEUGLY
64   VALPROGLYVALGLYGLYLEUGLYGLYILE
65   PROPROALAALAALAALALYSALAALALYS
66   TYRGLYALAALAGLYLEUGLYGLYVALLEU
67   GLYGLYALAGLYGLNPHEPROLEUGLYGLY
68   VALALAALAARGPROGLYPHEGLYLEUSER
69   PROILEPHEPROGLYGLYALACYSLEUGLY
70   LYSALACYSGLYARGLYSARGLYS

Samples:

sample_1: hTE monomer, [U-13C; U-15N; U-2H], 0.33 mM; sodium phosphate 50 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D NOESY-HSQCsample_1isotropicsample_conditions_1
3D HSQC-TOCSYsample_1isotropicsample_conditions_1
3D HCANsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HN(CO)Nsample_1isotropicsample_conditions_1
4D HNCOCAsample_1isotropicsample_conditions_1
4D HNCACOsample_1isotropicsample_conditions_1
4D HNcaNNHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks