BMRB Entry 53476

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53476

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Title:
Resonance assignments of 24-36 domains fragment of human tropoelastin (hTE)
Deposition date:
2025-12-09
Original release date:
2026-02-06
Authors:
Reichheld, Sean; Sharpe, Simon
Citation:

Citation: Reichheld, Sean; Muiznieks, Lisa; Liu, Zi Hao; Payliss, Brandon; Keeley, Fred; Sharpe, Simon. "A free energy landscape screen reveals the disordered conformational ensemble of tropoelastin"  .

Assembly members:

Assembly members:
entity_1, polymer, 231 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet32b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GVGLAPGVGVAPGVGVAPGV GVAPGIGPGGVAAAAKSAAK VAAKAQLRAAAGLGAGIPGL GVGVGVPGLGVGAGVPGLGV GAGVPGFGAVPGALAAAKAA KYGAAVPGVLGGLGALGGVG IPGGVVGAGPAAAAAAAKAA AKAAQFGLVGAAGLGGLGVG GLGVPGVGGLGGIPPAAAAK AAKYGAAGLGGVLGGAGQFP LGGVAARPGFGLSPIFPGGA CLGKACGRKRK

Data sets:
Data typeCount
13C chemical shifts629
15N chemical shifts207
1H chemical shifts656

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hTE_24-361

Entities:

Entity 1, hTE_24-36 231 residues - Formula weight is not available

1   GLYVALGLYLEUALAPROGLYVALGLYVAL
2   ALAPROGLYVALGLYVALALAPROGLYVAL
3   GLYVALALAPROGLYILEGLYPROGLYGLY
4   VALALAALAALAALALYSSERALAALALYS
5   VALALAALALYSALAGLNLEUARGALAALA
6   ALAGLYLEUGLYALAGLYILEPROGLYLEU
7   GLYVALGLYVALGLYVALPROGLYLEUGLY
8   VALGLYALAGLYVALPROGLYLEUGLYVAL
9   GLYALAGLYVALPROGLYPHEGLYALAVAL
10   PROGLYALALEUALAALAALALYSALAALA
11   LYSTYRGLYALAALAVALPROGLYVALLEU
12   GLYGLYLEUGLYALALEUGLYGLYVALGLY
13   ILEPROGLYGLYVALVALGLYALAGLYPRO
14   ALAALAALAALAALAALAALALYSALAALA
15   ALALYSALAALAGLNPHEGLYLEUVALGLY
16   ALAALAGLYLEUGLYGLYLEUGLYVALGLY
17   GLYLEUGLYVALPROGLYVALGLYGLYLEU
18   GLYGLYILEPROPROALAALAALAALALYS
19   ALAALALYSTYRGLYALAALAGLYLEUGLY
20   GLYVALLEUGLYGLYALAGLYGLNPHEPRO
21   LEUGLYGLYVALALAALAARGPROGLYPHE
22   GLYLEUSERPROILEPHEPROGLYGLYALA
23   CYSLEUGLYLYSALACYSGLYARGLYSARG
24   LYS

Samples:

sample_1: hTE fragment domains 18-26 monomer, [U-100% 13C; U-100% 15N], 1.3 mM; sodium phosphate 50 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D NOESY-HSQCsample_1isotropicsample_conditions_1
3D HSQC-TOCSYsample_1isotropicsample_conditions_1
3D HCANsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HN(CO)Nsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks