BMRB Entry 53475

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53475

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Title:
Resonance assignments of 18-26 domains fragment of human tropoelastin (hTE)
Deposition date:
2025-12-09
Original release date:
2026-02-06
Authors:
Reichheld, Sean; Sharpe, Simon
Citation:

Citation: Reichheld, Sean; Muiznieks, Lisa; Liu, Zi Hao; Payliss, Brandon; Keeley, Fred; Sharpe, Simon. "A free energy landscape screen reveals the disordered conformational ensemble of tropoelastin"  .

Assembly members:

Assembly members:
entity_1, polymer, 228 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet32b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AGVPGVGVPGAGIPVVPGAG IPGAAVPGVVSPEAAAKAAA KAAKYGARPGVGVGGIPTYG VGAGGFPGFGVGVGGIPGVA GVPGVGGVPGVGGVPGVGIS PEAQAAAAAKAAKYGVGTPA AAAAKAAAKAAQFGLVPGVG VAPGVGVAPGVGVAPGVGLA PGVGVAPGVGVAPGVGVAPG IGPGGVAAAAKSAAKVAAKA QLRAAAGLGAGIPGLGVGVG VPGLGVGA

Data sets:
Data typeCount
13C chemical shifts589
15N chemical shifts199
1H chemical shifts430

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hTE_18-261

Entities:

Entity 1, hTE_18-26 228 residues - Formula weight is not available

1   ALAGLYVALPROGLYVALGLYVALPROGLY
2   ALAGLYILEPROVALVALPROGLYALAGLY
3   ILEPROGLYALAALAVALPROGLYVALVAL
4   SERPROGLUALAALAALALYSALAALAALA
5   LYSALAALALYSTYRGLYALAARGPROGLY
6   VALGLYVALGLYGLYILEPROTHRTYRGLY
7   VALGLYALAGLYGLYPHEPROGLYPHEGLY
8   VALGLYVALGLYGLYILEPROGLYVALALA
9   GLYVALPROGLYVALGLYGLYVALPROGLY
10   VALGLYGLYVALPROGLYVALGLYILESER
11   PROGLUALAGLNALAALAALAALAALALYS
12   ALAALALYSTYRGLYVALGLYTHRPROALA
13   ALAALAALAALALYSALAALAALALYSALA
14   ALAGLNPHEGLYLEUVALPROGLYVALGLY
15   VALALAPROGLYVALGLYVALALAPROGLY
16   VALGLYVALALAPROGLYVALGLYLEUALA
17   PROGLYVALGLYVALALAPROGLYVALGLY
18   VALALAPROGLYVALGLYVALALAPROGLY
19   ILEGLYPROGLYGLYVALALAALAALAALA
20   LYSSERALAALALYSVALALAALALYSALA
21   GLNLEUARGALAALAALAGLYLEUGLYALA
22   GLYILEPROGLYLEUGLYVALGLYVALGLY
23   VALPROGLYLEUGLYVALGLYALA

Samples:

sample_1: hTE fragment domains 18-26 monomer, [U-100% 13C; U-100% 15N], 1.3 mM; sodium phosphate 50 mM; sodium chloride 200 mM

sample_conditions_1: ionic strength: 0.25 M; pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D NOESY-HSQCsample_1isotropicsample_conditions_1
3D HSQC-TOCSYsample_1isotropicsample_conditions_1
3D HCANsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HN(CO)Nsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks