BMRB Entry 53470

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53470

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Title:
EcMscL WT 13C detected
Deposition date:
2025-12-09
Original release date:
2026-09-01
Authors:
Kovinko, Alexandra; Shi, Chaowei; Lange, Adam
Citation:

Citation: Kovinko, Alexandra; Shi, Chaowei; Lange, Adam. "Atomic structure and dynamics of the mechanosensitive channel MscL from E. coli by cryo-EM and solid-state NMR "  Sci. Adv. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSIIKEFREFAMRGNVVDLA VGVIIGAAFGKIVSSLVADI IMPPLGLLIGGIDFKQFAVT LRDAQGDIPAVVMHYGVFIQ NVFDFLIVAFAIFMAIKLIN KLNRKKEEPAAAPAPTKEEV LLTEIRDLLKEQNNRSLEHH HHHH

Data sets:
Data typeCount
13C chemical shifts171
15N chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MscL1

Entities:

Entity 1, MscL 144 residues - Formula weight is not available

1   METSERILEILELYSGLUPHEARGGLUPHE
2   ALAMETARGGLYASNVALVALASPLEUALA
3   VALGLYVALILEILEGLYALAALAPHEGLY
4   LYSILEVALSERSERLEUVALALAASPILE
5   ILEMETPROPROLEUGLYLEULEUILEGLY
6   GLYILEASPPHELYSGLNPHEALAVALTHR
7   LEUARGASPALAGLNGLYASPILEPROALA
8   VALVALMETHISTYRGLYVALPHEILEGLN
9   ASNVALPHEASPPHELEUILEVALALAPHE
10   ALAILEPHEMETALAILELYSLEUILEASN
11   LYSLEUASNARGLYSLYSGLUGLUPROALA
12   ALAALAPROALAPROTHRLYSGLUGLUVAL
13   LEULEUTHRGLUILEARGASPLEULEULYS
14   GLUGLNASNASNARGSERLEUGLUHISHIS
15   HISHISHISHIS

Samples:

sample_1: DSS 3%; Azolectin 45 % w/w; HEPES 20 mM; potassium chloride 100 mM; MscL, [U-13C; U-15N], 45 % w/w

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 13C/13C PDSDsample_1anisotropicsample_conditions_1
2D NCAsample_1anisotropicsample_conditions_1
2D NCOsample_1anisotropicsample_conditions_1
3D NCACOsample_1anisotropicsample_conditions_1
3D NCOCAsample_1anisotropicsample_conditions_1
3D NCACXsample_1anisotropicsample_conditions_1
3D NCACBsample_1anisotropicsample_conditions_1
3D CONCAsample_1anisotropicsample_conditions_1
3D HCOCACBsample_1anisotropicsample_conditions_1
3D CANCOCAsample_1anisotropicsample_conditions_1
3D NCOCACBsample_1anisotropicsample_conditions_1
3D NCOCXsample_1anisotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment, peak picking

TOPSPIN v4 - collection, processing

NMR spectrometers:

  • Bruker Avance 700 MHz