BMRB Entry 53406

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53406

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Title:
1HN, 13C, and 15N resonance assignments of calcium-Calmodulin bound to the BP2 peptide derived from sheep STRA6, a vitamin A transporter
Deposition date:
2025-10-27
Original release date:
2026-02-05
Authors:
Cook, Mary; Varney, Kristen; Zhaung, Xinhao; Weber, David
Citation:

Citation: Cook, Mary; Varney, Kristen; Zhaung, Xinhao; Yazici, Aysenur; Mancia, Filippo; Weber, David. "1HN, 13C, and 15N resonance assignments of Ca2+-calmodulin bound to a BP2 peptide derived from the sheep STRA6 vitamin A transporter (residues 625-656)"  Biomol. NMR Assign. 20, 9-9 (2025).
PubMed: 41413324

Assembly members:

Assembly members:
entity_1, polymer, 148 residues, Formula weight is not available
entity_2, polymer, 32 residues, Formula weight is not available
entity_MG, non-polymer, 24.305 Da.
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
entity_2: RGAGPRARHGRARWGLAYTL LHNPALQAFRKT

Data sets:
Data typeCount
13C chemical shifts499
15N chemical shifts130
1H chemical shifts665

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin1
2BP22
3Mg2+3
4Ca2+4

Entities:

Entity 1, Calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, BP2 32 residues - Formula weight is not available

1   ARGGLYALAGLYPROARGALAARGHISGLY
2   ARGALAARGTRPGLYLEUALATYRTHRLEU
3   LEUHISASNPROALALEUGLNALAPHEARG
4   LYSTHR

Entity 3, Mg2+ - Mg - 24.305 Da.

1   MG

Entity 4, Ca2+ - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calmodulin, [U-13C; U-15N], 0.3 mM; BP2 1.2 mM; Magnesium 20 mM; Calcium 10 mM; HEPES 20 mM; sodium chloride 50 mM; TCEP 0.5 mM; sodium azide 0.34 mM

sample_conditions_1: ionic strength: 0.14 M; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection

TOPSPIN v3.6.5 - collection

NMRPipe - processing

CcpNMR v3.3 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks