BMRB Entry 53380

Name: Backbone resonance assignment of the CS-SGS region of yeast SGT1
Published: 2026-01-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53380

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignment of the CS-SGS region of yeast SGT1

Deposition date:

2025-10-03

Original release date:

2026-01-15

Authors:

Lopez, Abraham; Delhommel, Florent; Sattler, Michael

Citation:

Citation: Engler, Sonja; Delhommel, Florent; Dodt, Christopher; Lopez, Abraham; Faust, Ofrah; Elimelech, Annika; Napolitano, Valeria; Popowicz, Grzegorz; Rosenzweig, Rina; Sattler, Michael; Buchner, Johannes. "The essential co-chaperone Sgt1 regulates client dwell time in the Hsp90 chaperone cycle" Mol. Cell 86, 166-179 (2026).
PubMed: 41435837

Assembly members:

Assembly members:
entity_1, polymer, 212 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KFKIDWYQSSTSVTISLFTV NLPESKEQVNIYISPNDRRT LSISYQVPKSGSEFQYNAKL SHEVDPKAVSLKIFPKKLEI TLSKIDSTQWKKLEEDILTE SSRLSDEGKNSDSATRLLSA ETASKERLSYPSSSKKKIDW SKLDIDEEADEEAGSADSFF QKLYAGADPDTKRAMMKSFI ESNGTALSTDWEDVSKGTVK TSPPEGMEPKHW

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	558
15N chemical shifts	172
1H chemical shifts	172

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SGT1	1

Entities:

Entity 1, SGT1 212 residues - Formula weight is not available

1

LYS

PHE

LYS

ILE

ASP

TRP

TYR

GLN

SER

2

THR

SER

VAL

THR

ILE

SER

LEU

PHE

THR

VAL

3

ASN

LEU

PRO

GLU

SER

LYS

GLU

GLN

VAL

ASN

4

ILE

TYR

ILE

SER

PRO

ASN

ASP

ARG

THR

5

LEU

SER

ILE

SER

TYR

GLN

VAL

PRO

LYS

SER

6

GLY

SER

GLU

PHE

GLN

TYR

ASN

ALA

LYS

LEU

7

SER

HIS

GLU

VAL

ASP

PRO

LYS

ALA

VAL

SER

8

LEU

LYS

ILE

PHE

PRO

LYS

LEU

GLU

ILE

9

THR

LEU

SER

LYS

ILE

ASP

SER

THR

GLN

TRP

10

LYS

LEU

GLU

ASP

ILE

LEU

THR

GLU

11

SER

ARG

LEU

SER

ASP

GLU

GLY

LYS

ASN

12

SER

ASP

SER

ALA

THR

ARG

LEU

SER

ALA

13

GLU

THR

ALA

SER

LYS

GLU

ARG

LEU

SER

TYR

14

PRO

SER

LYS

ILE

ASP

TRP

15

SER

LYS

LEU

ASP

ILE

ASP

GLU

ALA

ASP

16

GLU

ALA

GLY

SER

ALA

ASP

SER

PHE

17

GLN

LYS

LEU

TYR

ALA

GLY

ALA

ASP

PRO

ASP

18

THR

LYS

ARG

ALA

MET

LYS

SER

PHE

ILE

19

GLU

SER

ASN

GLY

THR

ALA

LEU

SER

THR

ASP

20

TRP

GLU

ASP

VAL

SER

LYS

GLY

THR

VAL

LYS

21

THR

SER

PRO

GLU

GLY

MET

GLU

PRO

LYS

22

HIS

TRP

Samples:

sample_1: SGT1 CS-SGS, [U-100% 13C; U-100% 15N], 550 uM; Phosphate 50 mM; NaCl 100 mM; DTT 5 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - data analysis

NMRPipe - processing

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE III 600 MHz