BMRB Entry 53375

Name: assignment of FliD40
Published: 2025-11-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53375

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

assignment of FliD40

Deposition date:

2025-09-27

Original release date:

2025-11-22

Authors:

Xing, Qiong

Citation:

Citation: Chen, LiXia; Cheng, Xiaoqi; Zhang, Fangfang; Wang, Xin; Jiang, Wenxue; Ma, Lixin; Xing, Qiong. "Ultraweak interactions drive cap-mediated positioning and elongation of the bacterial flagellar filament" Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 182 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAHMNSAKLTAYGTLKSALE KFQTANTALNKADLFKSDTK AKEAIKSWVDAYNSLVDTFS SLTKYTAVEPGEEASDKNGA LLGDSVVRTIQTGIRAQFAN SGSNSAFKTMAEIGITQDGT SGKLKIDDDKLTKVLKDNTA AARELLVGDGKETGITTKIA TEVKSYLADDGIIDNAQDNV NA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	238
15N chemical shifts	117
1H chemical shifts	116

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FliD	1

Entities:

Entity 1, FliD 182 residues - Formula weight is not available

1

GLY

ALA

HIS

MET

ASN

SER

ALA

LYS

LEU

THR

2

ALA

TYR

GLY

THR

LEU

LYS

SER

ALA

LEU

GLU

3

LYS

PHE

GLN

THR

ALA

ASN

THR

ALA

LEU

ASN

4

LYS

ALA

ASP

LEU

PHE

LYS

SER

ASP

THR

LYS

5

ALA

LYS

GLU

ALA

ILE

LYS

SER

TRP

VAL

ASP

6

ALA

TYR

ASN

SER

LEU

VAL

ASP

THR

PHE

SER

7

SER

LEU

THR

LYS

TYR

THR

ALA

VAL

GLU

PRO

8

GLY

GLU

ALA

SER

ASP

LYS

ASN

GLY

ALA

9

LEU

GLY

ASP

SER

VAL

ARG

THR

ILE

10

GLN

THR

GLY

ILE

ARG

ALA

GLN

PHE

ALA

ASN

11

SER

GLY

SER

ASN

SER

ALA

PHE

LYS

THR

MET

12

ALA

GLU

ILE

GLY

ILE

THR

GLN

ASP

GLY

THR

13

SER

GLY

LYS

LEU

LYS

ILE

ASP

LYS

14

LEU

THR

LYS

VAL

LEU

LYS

ASP

ASN

THR

ALA

15

ALA

ARG

GLU

LEU

VAL

GLY

ASP

GLY

16

LYS

GLU

THR

GLY

ILE

THR

LYS

ILE

ALA

17

THR

GLU

VAL

LYS

SER

TYR

LEU

ALA

ASP

18

GLY

ILE

ASP

ASN

ALA

GLN

ASP

ASN

VAL

19

ASN

ALA

Samples:

sample_1: FliD, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 25 mM; NaCl 0.15 M

sample_conditions_1: ionic strength: 0.15 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks