BMRB Entry 53342

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53342

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Title:
1H, 13C and 15N backbone resonance assignment of the lytic polysaccharide monooxygenase LsAA9A from Lentinus similis
Deposition date:
2025-09-08
Original release date:
2025-12-20
Authors:
Wiesinger, Piera; Sandgren, Mats; Nestor, Gustav
Citation:

Citation: Wiesinger, Piera; Sandgren, Mats; Nestor, Gustav. "1H, 13C and 15N backbone resonance assignment of the lytic polysaccharide monooxygenase LsAA9A from Lentinus similis"  Biomol. NMR Assign. 20, 5-5 (2025).
PubMed: 41286536

Assembly members:

Assembly members:
entity_1, polymer, 235 residues, 25241 Da.

Natural source:

Natural source:   Common Name: Lentinus similis   Taxonomy ID: 292560   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Lentinus similis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLyGo-Ec-6

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: HTLVWGVWVNGVDQGDGRNI YIRSPPNNNPVKNLTSPDMT CNVDNRVVPKSVPVNAGDTL TFEWYHNTRDDDIIASSHHG PIAVYIAPAASNGQGNVWVK LFEDAYNVTNSTWAVDRLIT AHGQHSVVVPHVAPGDYLFR AEIIALHEADSLYSQNPIRG AQFYISCAQITINSSDDSTP LPAGVPFPGAYTDSTPGIQF NIYTTPATSYVAPPPSVWSG ALGGSIAQVGDASLE

Data sets:
Data typeCount
13C chemical shifts648
15N chemical shifts208
1H chemical shifts664

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LsAA9A1

Entities:

Entity 1, LsAA9A 235 residues - 25241 Da.

1   HISTHRLEUVALTRPGLYVALTRPVALASN
2   GLYVALASPGLNGLYASPGLYARGASNILE
3   TYRILEARGSERPROPROASNASNASNPRO
4   VALLYSASNLEUTHRSERPROASPMETTHR
5   CYSASNVALASPASNARGVALVALPROLYS
6   SERVALPROVALASNALAGLYASPTHRLEU
7   THRPHEGLUTRPTYRHISASNTHRARGASP
8   ASPASPILEILEALASERSERHISHISGLY
9   PROILEALAVALTYRILEALAPROALAALA
10   SERASNGLYGLNGLYASNVALTRPVALLYS
11   LEUPHEGLUASPALATYRASNVALTHRASN
12   SERTHRTRPALAVALASPARGLEUILETHR
13   ALAHISGLYGLNHISSERVALVALVALPRO
14   HISVALALAPROGLYASPTYRLEUPHEARG
15   ALAGLUILEILEALALEUHISGLUALAASP
16   SERLEUTYRSERGLNASNPROILEARGGLY
17   ALAGLNPHETYRILESERCYSALAGLNILE
18   THRILEASNSERSERASPASPSERTHRPRO
19   LEUPROALAGLYVALPROPHEPROGLYALA
20   TYRTHRASPSERTHRPROGLYILEGLNPHE
21   ASNILETYRTHRTHRPROALATHRSERTYR
22   VALALAPROPROPROSERVALTRPSERGLY
23   ALALEUGLYGLYSERILEALAGLNVALGLY
24   ASPALASERLEUGLU

Samples:

sample_1: LsAA9A, [U-13C; U-15N], 300 uM; D2O, [U-99% 2H], 10%; sodium phosphate 40 mM; sodium chloride 10 mM; sodium azide 0.02%; DSS 0.5 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.1.4 - collection, processing

CcpNMR v3 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks