BMRB Entry 53284
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53284
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Citation: Lalic, Milena; Thomsen, Elisabeth; Thomasen, F.; Udupa, Aditya; Andersen, Emma; Kuppermann, Sonja; Christensen, Casper; Holm-Olesen, Emma; Saar, Daniel; Prestel, Andreas; Bauer, Lukas; McKenna, Joseph; Olsen, Johan; Mandrup, Susanne; Hartmann-Petersen, Rasmus; Jeffries, Cy; Staller, Max; Lindorff-Larsen, Kresten; Kragelund, Birthe. "Autoinhibition of untimely DNA binding by structural disorder in the full-length peroxisome proliferator-activated receptors (PPARs)
" Nat. Commun. ., .-. (2026).
Assembly members:
entity_1, polymer, 116 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Modified pET-24b
Entity Sequences (FASTA):
entity_1: GPPLTTTLPACCGPIARCLT
SPQAYENQEQLKDDDSDLIL
NDGDISLTYGDSTVNTEPAT
SSAPRRFMGNSSEDALDREL
AFGDHELVIRGTRLVLPMDD
SEPPLNLLDNTRHGPA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 326 |
| 15N chemical shifts | 104 |
| 1H chemical shifts | 104 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NHE6 C-terminal | 1 |
Entities:
Entity 1, NHE6 C-terminal 116 residues - Formula weight is not available
| 1 | GLY | PRO | PRO | LEU | THR | THR | THR | LEU | PRO | ALA | ||||
| 2 | CYS | CYS | GLY | PRO | ILE | ALA | ARG | CYS | LEU | THR | ||||
| 3 | SER | PRO | GLN | ALA | TYR | GLU | ASN | GLN | GLU | GLN | ||||
| 4 | LEU | LYS | ASP | ASP | ASP | SER | ASP | LEU | ILE | LEU | ||||
| 5 | ASN | ASP | GLY | ASP | ILE | SER | LEU | THR | TYR | GLY | ||||
| 6 | ASP | SER | THR | VAL | ASN | THR | GLU | PRO | ALA | THR | ||||
| 7 | SER | SER | ALA | PRO | ARG | ARG | PHE | MET | GLY | ASN | ||||
| 8 | SER | SER | GLU | ASP | ALA | LEU | ASP | ARG | GLU | LEU | ||||
| 9 | ALA | PHE | GLY | ASP | HIS | GLU | LEU | VAL | ILE | ARG | ||||
| 10 | GLY | THR | ARG | LEU | VAL | LEU | PRO | MET | ASP | ASP | ||||
| 11 | SER | GLU | PRO | PRO | LEU | ASN | LEU | LEU | ASP | ASN | ||||
| 12 | THR | ARG | HIS | GLY | PRO | ALA |
Samples:
sample_1: NHE6 C-terminal, [U-100% 13C; U-100% 15N], 500 uM
sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 278.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)NNH | sample_1 | isotropic | sample_conditions_1 |
Software:
ANALYSIS - chemical shift assignment
NMR spectrometers:
- Bruker Avance 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks