BMRB Entry 53283

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53283
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
Fd3
Deposition date:
2025-07-15
Original release date:
2026-06-25
Authors:
Srb, Pavel; Giacobelli, Valerio; Veverka, Vaclav
Citation:

Citation: Srb, Pavel. "Ancient amino acid sets enable stable protein folds "  .

Assembly members:

Assembly members:
entity_1, polymer, 75 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MATTITISGDAALLAEALEE AEALLAAGVIDAVEVVDGAL VITVAPADAEAVAEELAEAV PGITVEISAHHHHHH

Data sets:
Data typeCount
13C chemical shifts261
15N chemical shifts61
1H chemical shifts444

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fd31

Entities:

Entity 1, Fd3 75 residues - Formula weight is not available

1   METALATHRTHRILETHRILESERGLYASP
2   ALAALALEULEUALAGLUALALEUGLUGLU
3   ALAGLUALALEULEUALAALAGLYVALILE
4   ASPALAVALGLUVALVALASPGLYALALEU
5   VALILETHRVALALAPROALAASPALAGLU
6   ALAVALALAGLUGLULEUALAGLUALAVAL
7   PROGLYILETHRVALGLUILESERALAHIS
8   HISHISHISHISHIS

Samples:

sample_1: Fd3, [U-100% 13C; U-100% 15N], 200 ± 30 uM

sample_conditions_1: ionic strength: 0.33 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 15N-separated NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6.5 - collection

POKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks