BMRB Entry 53248

Name: ctCsl4 amide backbone assignments
Published: 2025-07-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53248

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

ctCsl4 amide backbone assignments

Deposition date:

2025-06-27

Original release date:

2025-07-08

Authors:

Liebau, Jobst; Sprangers, Remco

Citation:

Citation: Liebau, Jobst; Lazzaretti, Daniela; Furtges, Torben; Bichler, Anna; Pilsl, Michael; Rudack, Till; Sprangers, Remco. "4D structural biology-quantitative dynamics in the eukaryotic RNA exosome complex" Nat. Commun. 16, 7896-7896 (2025).
PubMed: 40849410

Assembly members:

Assembly members:
entity_1, polymer, 222 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Chaetomium thermophilum Taxonomy ID: 209285 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Chaetomium thermophilum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMTTTQPTLALPGQLLGPI SKYQPGPGTHVHESNLYSSL LGTVHVTQPARAPGPVKRLN RITPAPTPAELPTISVSAAR PAGSAASGLVTGRKREILPE VGNIVLCRVIRITPRQAVVT ILVCGDTVLDAEWQGLIRVQ DIRATEKDRVKVYESFRPGD IVRAEVISLGDQANYYLSTA RNELGVILATSEAGNTMYPV SWREYRDPITGLTELRKVAK PY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	137
1H chemical shifts	137

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Csl4	1

Entities:

Entity 1, Csl4 222 residues - Formula weight is not available

1

GLY

ALA

MET

THR

GLN

PRO

THR

LEU

2

ALA

LEU

PRO

GLY

GLN

LEU

GLY

PRO

ILE

3

SER

LYS

TYR

GLN

PRO

GLY

PRO

GLY

THR

HIS

4

VAL

HIS

GLU

SER

ASN

LEU

TYR

SER

LEU

5

LEU

GLY

THR

VAL

HIS

VAL

THR

GLN

PRO

ALA

6

ARG

ALA

PRO

GLY

PRO

VAL

LYS

ARG

LEU

ASN

7

ARG

ILE

THR

PRO

ALA

PRO

THR

PRO

ALA

GLU

8

LEU

PRO

THR

ILE

SER

VAL

SER

ALA

ARG

9

PRO

ALA

GLY

SER

ALA

SER

GLY

LEU

VAL

10

THR

GLY

ARG

LYS

ARG

GLU

ILE

LEU

PRO

GLU

11

VAL

GLY

ASN

ILE

VAL

LEU

CYS

ARG

VAL

ILE

12

ARG

ILE

THR

PRO

ARG

GLN

ALA

VAL

THR

13

ILE

LEU

VAL

CYS

GLY

ASP

THR

VAL

LEU

ASP

14

ALA

GLU

TRP

GLN

GLY

LEU

ILE

ARG

VAL

GLN

15

ASP

ILE

ARG

ALA

THR

GLU

LYS

ASP

ARG

VAL

16

LYS

VAL

TYR

GLU

SER

PHE

ARG

PRO

GLY

ASP

17

ILE

VAL

ARG

ALA

GLU

VAL

ILE

SER

LEU

GLY

18

ASP

GLN

ALA

ASN

TYR

LEU

SER

THR

ALA

19

ARG

ASN

GLU

LEU

GLY

VAL

ILE

LEU

ALA

THR

20

SER

GLU

ALA

GLY

ASN

THR

MET

TYR

PRO

VAL

21

SER

TRP

ARG

GLU

TYR

ARG

ASP

PRO

ILE

THR

22

GLY

LEU

THR

GLU

LEU

ARG

LYS

VAL

ALA

LYS

23

PRO

TYR

Samples:

sample_1: Csl4, [U-100% 13C; U-100% 15N; U-80% 2H], 360 uM; HEPES 25 mM; NaCl 200 mM; DTT 1 mM

sample_conditions_1: ionic strength: 200 mM; pH: 7.5; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1.7 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz