BMRB Entry 53246

Name: A human ATG3 deletion construct (D89-192)
Published: 2025-07-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53246

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

A human ATG3 deletion construct (D89-192)

Deposition date:

2025-06-26

Original release date:

2025-07-29

Authors:

Ohashi, Kazuto; Otomo, Takanori

Citation:

Citation: Ohashi, Kazuto; Kroon, Gerard; Otomo, Takanori. "Structural Insights into the GABARAP-ATG3 Backside Interaction and Apo ATG3 Conformation" Biochemistry 64, 3178-3189 (2025).
PubMed: 40628661

Assembly members:

Assembly members:
entity_1, polymer, 215 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMAMAQNVINTVKGKALEV AEYLTPVLKESKFKETGVIT PEEFVAAGDHLVHHCPTWQW ATGEELKVKAYLPTGKQFLV TKNVPCYKRCKQMAILQTRT YDLYITYDKYYQTPRLWLFG YDEQRQPLTVEHMYEDISQD HVKKTVTIENHPHLPPPPMC SVHPCRHAEVMKKIIETVAE GGGELGVHMYLLIFLKFVQA VIPTIEYDYTRHFTM

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	550
15N chemical shifts	175
1H chemical shifts	175

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ATG3 monomer	1

Entities:

Entity 1, ATG3 monomer 215 residues - Formula weight is not available

GHMAMAQ+(ATG3 residues 2-188+193-314)

1

GLY

HIS

MET

ALA

MET

ALA

GLN

ASN

VAL

ILE

2

ASN

THR

VAL

LYS

GLY

LYS

ALA

LEU

GLU

VAL

3

ALA

GLU

TYR

LEU

THR

PRO

VAL

LEU

LYS

GLU

4

SER

LYS

PHE

LYS

GLU

THR

GLY

VAL

ILE

THR

5

PRO

GLU

PHE

VAL

ALA

GLY

ASP

HIS

6

LEU

VAL

HIS

CYS

PRO

THR

TRP

GLN

TRP

7

ALA

THR

GLY

GLU

LEU

LYS

VAL

LYS

ALA

8

TYR

LEU

PRO

THR

GLY

LYS

GLN

PHE

LEU

VAL

9

THR

LYS

ASN

VAL

PRO

CYS

TYR

LYS

ARG

CYS

10

LYS

GLN

MET

ALA

ILE

LEU

GLN

THR

ARG

THR

11

TYR

ASP

LEU

TYR

ILE

THR

TYR

ASP

LYS

TYR

12

TYR

GLN

THR

PRO

ARG

LEU

TRP

LEU

PHE

GLY

13

TYR

ASP

GLU

GLN

ARG

GLN

PRO

LEU

THR

VAL

14

GLU

HIS

MET

TYR

GLU

ASP

ILE

SER

GLN

ASP

15

HIS

VAL

LYS

THR

VAL

THR

ILE

GLU

ASN

16

HIS

PRO

HIS

LEU

PRO

MET

CYS

17

SER

VAL

HIS

PRO

CYS

ARG

HIS

ALA

GLU

VAL

18

MET

LYS

ILE

GLU

THR

VAL

ALA

GLU

19

GLY

GLU

LEU

GLY

VAL

HIS

MET

TYR

20

LEU

ILE

PHE

LEU

LYS

PHE

VAL

GLN

ALA

21

VAL

ILE

PRO

THR

ILE

GLU

TYR

ASP

TYR

THR

22

ARG

HIS

PHE

THR

MET

Samples:

sample_1: ATG3 double-labeled, [U-100% 13C; U-100% 15N], 1.2 M

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw - processing

NMRPipe - processing

NMRViewJ - chemical shift assignment, peak picking

NMR spectrometers:

Bruker DRX 600 MHz