Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53243

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

ZGRF1-DUF2439

Deposition date:

2025-06-25

Original release date:

2026-10-05

Authors:

Staby, Lasse; Simonsen, Anne Katrine; Lisby, Michael; Kragelund, Birthe

Citation:

Citation: Staby, Lasse; Simonsen, Anne Katrine; Lisby, Michael; Kragelund, Birthe. "The DUF2439 domain of ZGRF1 is a RAD51 interactor and facilitator " .

Assembly members:

Assembly members:
entity_1, polymer, 99 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-3X

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GIPMESQEFIVLYTHQKMKK SKVWQDGILKITHLGNKAIL YDDKGACLESLFLKCLEVKP GDDLESDRYLITVEEVKVAG AIGIVKQNVNKEAPELNSR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	236
15N chemical shifts	72
1H chemical shifts	247

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DUF2439	1

Entities:

Entity 1, DUF2439 99 residues - Formula weight is not available

Residues 1-3 (GIP) represent non-native residues leftover after cleavage.

1

GLY

ILE

PRO

MET

GLU

SER

GLN

GLU

PHE

ILE

2

VAL

LEU

TYR

THR

HIS

GLN

LYS

MET

LYS

3

SER

LYS

VAL

TRP

GLN

ASP

GLY

ILE

LEU

LYS

4

ILE

THR

HIS

LEU

GLY

ASN

LYS

ALA

ILE

LEU

5

TYR

ASP

LYS

GLY

ALA

CYS

LEU

GLU

SER

6

LEU

PHE

LEU

LYS

CYS

LEU

GLU

VAL

LYS

PRO

7

GLY

ASP

LEU

GLU

SER

ASP

ARG

TYR

LEU

8

ILE

THR

VAL

GLU

VAL

LYS

VAL

ALA

GLY

9

ALA

ILE

GLY

ILE

VAL

LYS

GLN

ASN

VAL

ASN

10

LYS

GLU

ALA

PRO

GLU

LEU

ASN

SER

ARG

Samples:

sample_1: ZGRF1 DUF2439, [U-100% 13C; U-100% 15N], 80 uM; sodium chloride 150 mM; TRIS 25 mM; TCEP 2 mM; DSS 0.25 mM

sample_2: ZGRF1 DUF2439, [U-100% 15N], 80 uM; sodium chloride 150 mM; TRIS 25 mM; TCEP 2 mM; DSS 0.25 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
T1/R1 relaxation	sample_2	isotropic	sample_conditions_1
T1/R1 relaxation	sample_2	isotropic	sample_conditions_1
T2/R2 relaxation	sample_2	isotropic	sample_conditions_1
T2/R2 relaxation	sample_2	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment, data analysis, peak picking

TOPSPIN - collection

NMRDraw - processing

MddNMR - processing

NMR spectrometers:

Bruker Avance 750 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 53243