BMRB Entry 53235
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53235
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Gupta, Varsha; Laugwitz, Jeannette; Sklodowski, Mateusz; Voitel, Matthias; Sala, Davide; Kaiser, Anette; Elgeti, Matthias; Meiler, Jens; Huster, Daniel; Beck-Sickinger, Annette; Schmidt, Peter; Penk, Anja. "Structural insights into the binding modes of Arrestin-3 finger loop to the neuropeptide Y1 and Y2 receptors" Structure 33, 1728-1738 (2025).
PubMed: 40780192
Assembly members:
entity_1, polymer, 17 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: AFRYGREDLDVLGLSFR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 30 |
| 1H chemical shifts | 18 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | finger loop peptide | 1 |
Entities:
Entity 1, finger loop peptide 17 residues - Formula weight is not available
| 1 | ALA | PHE | ARG | TYR | GLY | ARG | GLU | ASP | LEU | ASP | ||||
| 2 | VAL | LEU | GLY | LEU | SER | PHE | ARG |
Samples:
sample_1: finger loop peptide, [U-100% 13C; U-100% 15N], 190 uM; NaP 50 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 700 MHz