BMRB Entry 53234

Name: The NMR of smt3(yeast sumo) reacting with Cu(II)
Published: 2025-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53234

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The NMR of smt3(yeast sumo) reacting with Cu(II)

Deposition date:

2025-06-17

Original release date:

2025-06-30

Authors:

Xiang, Sijia; Liu, Yangzhong; Cao, Kaiming

Citation:

Citation: Xiang, Sijia; Hou, Zhuanghao; Wang, Yu; Yang, Yang; Hu, Hongze; Yin, Chang; Huang, Guangming; Cao, Kaiming; Liu, Yangzhong. "Metal-induced nanoscale clusterization initiates protein liquid-liquid phase separation" Cell Rep. 44, 116067-116067 (2025).
PubMed: 40716064

Assembly members:

Assembly members:
entity_1, polymer, 101 residues, Formula weight is not available
entity_CU, non-polymer, 63.546 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMSDSEVNQEAKPEVKPEVK PETHINLKVSDGSSEIFFKI KKTTPLRRLMEAFAKRQGKE MDSLRFLYDGIRIQADQTPE DLDMEDNDIIEAHREQIGGH M

Data sets:

Data type

Count

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sumo	1
2	Cu	2

Entities:

Entity 1, sumo 101 residues - Formula weight is not available

1

SER

MET

SER

ASP

SER

GLU

VAL

ASN

GLN

GLU

2

ALA

LYS

PRO

GLU

VAL

LYS

PRO

GLU

VAL

LYS

3

PRO

GLU

THR

HIS

ILE

ASN

LEU

LYS

VAL

SER

4

ASP

GLY

SER

GLU

ILE

PHE

LYS

ILE

5

LYS

THR

PRO

LEU

ARG

LEU

MET

6

GLU

ALA

PHE

ALA

LYS

ARG

GLN

GLY

LYS

GLU

7

MET

ASP

SER

LEU

ARG

PHE

LEU

TYR

ASP

GLY

8

ILE

ARG

ILE

GLN

ALA

ASP

GLN

THR

PRO

GLU

9

ASP

LEU

ASP

MET

GLU

ASP

ASN

ASP

ILE

10

GLU

ALA

HIS

ARG

GLU

GLN

ILE

GLY

HIS

11

MET

Entity 2, Cu - Cu - 63.546 Da.

1

CU

Samples:

sample_1: smt3, [U-100% 15N], 100 uM; Divalent copper ion 150 uM; phosphate buffer 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 15N	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE III 600 MHz