BMRB Entry 53208

Name: Backbone assignment of the the complex between the C-terminal domain of the OSCP subunit and a peptide mimicking the C-terminal helix of the b subunit of human ATP Synthase
Published: 2025-11-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53208

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of the the complex between the C-terminal domain of the OSCP subunit and a peptide mimicking the C-terminal helix of the b subunit of human ATP Synthase

Deposition date:

2025-05-28

Original release date:

2025-11-08

Authors:

Fabbian, Simone

Citation:

Citation: Fabbian, Simone; Gabbatore, Laura; Morbiato, Laura; de Zotti, Marta; Giorgio, Valentina; Battistutta, Roberto; Giachin, Gabriele; Sosic, Alice; Bellanda, Massimo. "The OSCP Subunit of ATP Synthase is a Dimer in Solution: Strategy to Induce the Monomeric Protein as a New Tool for Drug Discovery" J. Mol. Biol. 437, 169267-169267 (2025).
PubMed: 40482958

Assembly members:

Assembly members:
entity_1, polymer, 77 residues, Formula weight is not available
entity_2, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: ChampionTM pET SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GEVPCTVTSASPLEEATLSE LKTVLKSFLSQGQVLKLEAK TDPSILGGMIVRIGEKYVDM SVKTKIQKLGRAMREIV
entity_2: EKETIAKCIADLKLLAKKAN AN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	189
15N chemical shifts	73
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	OSCP_CT	1
2	C-terminal helix of b subunit	2

Entities:

Entity 1, OSCP_CT 77 residues - Formula weight is not available

1

GLY

GLU

VAL

PRO

CYS

THR

VAL

THR

SER

ALA

2

SER

PRO

LEU

GLU

ALA

THR

LEU

SER

GLU

3

LEU

LYS

THR

VAL

LEU

LYS

SER

PHE

LEU

SER

4

GLN

GLY

GLN

VAL

LEU

LYS

LEU

GLU

ALA

LYS

5

THR

ASP

PRO

SER

ILE

LEU

GLY

MET

ILE

6

VAL

ARG

ILE

GLY

GLU

LYS

TYR

VAL

ASP

MET

7

SER

VAL

LYS

THR

LYS

ILE

GLN

LYS

LEU

GLY

8

ARG

ALA

MET

ARG

GLU

ILE

VAL

Entity 2, C-terminal helix of b subunit 22 residues - Formula weight is not available

1

GLU

LYS

GLU

THR

ILE

ALA

LYS

CYS

ILE

ALA

2

ASP

LEU

LYS

LEU

ALA

LYS

ALA

ASN

3

ALA

ASN

Samples:

sample_1: OSCP_CT, [U-13C; U-15N], 1 mM; bCT peptide 10 mM; phosphate 50 mM; L-glutamic acid 50 mM; L-arginine 50 mM; DTT 2 mM

sample_conditions_1: ionic strength: 250 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

Bruker Topspin v4.0.6 - processing

CARA v1.9 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz