BMRB Entry 53204

Name: Backbone assignment of the N-terminal domain of the human ATP Synthase OSCP subunit.
Published: 2025-11-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53204

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of the N-terminal domain of the human ATP Synthase OSCP subunit.

Deposition date:

2025-05-27

Original release date:

2025-11-08

Authors:

Fabbian, Simone

Citation:

Citation: Fabbian, Simone; Gabbatore, Laura; Morbiato, Laura; de Zotti, Marta; Giorgio, Valentina; Battistutta, Roberto; Giachin, Gabriele; Sosic, Alice; Bellanda, Massimo. "The OSCP Subunit of ATP Synthase is a Dimer in Solution: Strategy to Induce the Monomeric Protein as a New Tool for Drug Discovery" J. Mol. Biol. 437, 169267-169267 (2025).
PubMed: 40482958

Assembly members:

Assembly members:
entity_1, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: ChampionTM pET SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RPPVQVYGIEGRYATALYSA ASKQNKLEQVEKELLRVAQI LKEPKVAASVLNPYVKRSIK VKSLNDITAKERFSPLTTNL INLLAENGRLSNTQGVVSAF STMMSVHRG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	246
15N chemical shifts	102
1H chemical shifts	102

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	OSCP_NT	1

Entities:

Entity 1, OSCP_NT 109 residues - Formula weight is not available

1

ARG

PRO

VAL

GLN

VAL

TYR

GLY

ILE

GLU

2

GLY

ARG

TYR

ALA

THR

ALA

LEU

TYR

SER

ALA

3

ALA

SER

LYS

GLN

ASN

LYS

LEU

GLU

GLN

VAL

4

GLU

LYS

GLU

LEU

ARG

VAL

ALA

GLN

ILE

5

LEU

LYS

GLU

PRO

LYS

VAL

ALA

SER

VAL

6

LEU

ASN

PRO

TYR

VAL

LYS

ARG

SER

ILE

LYS

7

VAL

LYS

SER

LEU

ASN

ASP

ILE

THR

ALA

LYS

8

GLU

ARG

PHE

SER

PRO

LEU

THR

ASN

LEU

9

ILE

ASN

LEU

ALA

GLU

ASN

GLY

ARG

LEU

10

SER

ASN

THR

GLN

GLY

VAL

SER

ALA

PHE

11

SER

THR

MET

SER

VAL

HIS

ARG

GLY

Samples:

sample_1: OSCP_NT, [U-13C; U-15N], 0.5 mM; phosphate 50 mM; L-glutamic acid 50 mM; L-arginine 50 mM; NaCl 200 mM

sample_conditions_1: ionic strength: 450 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

Bruker Topspin v4.0.6 - processing

CARA v1.9 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks