BMRB Entry 53190

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53190
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
NMR assignments for MKRN3-CH
Deposition date:
2025-05-17
Original release date:
2026-03-12
Authors:
Rua, Antonio; Alexandrescu, Andrei
Citation:

Citation: Rua, Antonio; Alexandrescu, Andrei. "Solution structure of the novel CH-domain zinc finger from the puberty regulator makorin-3"  Biochem. Biophys. Res. Commun. 811, 153579-153579 (2026).

Assembly members:

Assembly members:
entity_1, polymer, 31 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: solid-phase peptide synthesis/obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DICDMCGLQTLHPMDAAQRE EHMRACIEAHE

Data sets:
Data typeCount
13C chemical shifts85
15N chemical shifts32
1H chemical shifts185

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MKRN3-CH1
2Zn2

Entities:

Entity 1, MKRN3-CH 31 residues - Formula weight is not available

1   ASPILECYSASPMETCYSGLYLEUGLNTHR
2   LEUHISPROMETASPALAALAGLNARGGLU
3   GLUHISMETARGALACYSILEGLUALAHIS
4   GLU

Entity 2, Zn - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: MKRN3-CH 1.9 mM; Zn 2.3 mM; DSS 1 mM; D2O 10%; H2O 90%

sample_2: ZFHX3-CH 2.2 mM; Zn 2.5 mM; DSS 1 mM; D2O 100%

sample_3: ZFHX3-CH 1.9 mM; Zn 2.3 mM; DSS 1 mM; D2O 100%

sample_conditions_1: ionic strength: 1.9 mM; pH: 6.0; pressure: 1 atm; temperature: 280 K

sample_conditions_2: ionic strength: 1.9 mM; pH: 10.8; pressure: 1 atm; temperature: 280 K

sample_conditions_3: ionic strength: 1.9 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

sample_conditions_4: ionic strength: 2.2 mM; pH: 6.2; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_4
2D 1H-1H NOESYsample_2isotropicsample_conditions_4
2D 1H-15N HSQCsample_1isotropicsample_conditions_3
2D 1H-13C HSQCsample_3isotropicsample_conditions_4
2D 1H-1H TOCSYsample_1isotropicsample_conditions_2

Software:

CcpNMR - chemical shift assignment, peak picking

ARIA2 - refinement

PSVS - data analysis

TALOS-N - geometry optimization

X-PLOR NIH - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker AVANCE NEO 800 MHz
  • Bruker AVANCE NEO 600 MHz

Related Database Links:

UNP Q13064

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks