BMRB Entry 53126
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53126
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: von Ehr, Julian; Schlundt, Andreas. "1H, 13C, 15N backbone chemical shift assignments and relaxation analysis of the single and tandem RRMs of the human serine-arginine rich splicing factor 6 (SRSF6)" J. Magn. Reson. ., .-..
Assembly members:
entity_1, polymer, 188 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: JvE051
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 473 |
| 15N chemical shifts | 169 |
| 1H chemical shifts | 476 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SRSF6_1-188 | 1 |
Entities:
Entity 1, SRSF6_1-188 188 residues - Formula weight is not available
| 1 | MET | PRO | ARG | VAL | TYR | ILE | GLY | ARG | LEU | SER | ||||
| 2 | TYR | ASN | VAL | ARG | GLU | LYS | ASP | ILE | GLN | ARG | ||||
| 3 | PHE | PHE | SER | GLY | TYR | GLY | ARG | LEU | LEU | GLU | ||||
| 4 | VAL | ASP | LEU | LYS | ASN | GLY | TYR | GLY | PHE | VAL | ||||
| 5 | GLU | PHE | GLU | ASP | SER | ARG | ASP | ALA | ASP | ASP | ||||
| 6 | ALA | VAL | TYR | GLU | LEU | ASN | GLY | LYS | GLU | LEU | ||||
| 7 | CYS | GLY | GLU | ARG | VAL | ILE | VAL | GLU | HIS | ALA | ||||
| 8 | ARG | GLY | PRO | ARG | ARG | ASP | ARG | ASP | GLY | TYR | ||||
| 9 | SER | TYR | GLY | SER | ARG | SER | GLY | GLY | GLY | GLY | ||||
| 10 | TYR | SER | SER | ARG | ARG | THR | SER | GLY | ARG | ASP | ||||
| 11 | LYS | TYR | GLY | PRO | PRO | VAL | ARG | THR | GLU | TYR | ||||
| 12 | ARG | LEU | ILE | VAL | GLU | ASN | LEU | SER | SER | ARG | ||||
| 13 | CYS | SER | TRP | GLN | ASP | LEU | LYS | ASP | PHE | MET | ||||
| 14 | ARG | GLN | ALA | GLY | GLU | VAL | THR | TYR | ALA | ASP | ||||
| 15 | ALA | HIS | LYS | GLU | ARG | THR | ASN | GLU | GLY | VAL | ||||
| 16 | ILE | GLU | PHE | ARG | SER | TYR | SER | ASP | MET | LYS | ||||
| 17 | ARG | ALA | LEU | ASP | LYS | LEU | ASP | GLY | THR | GLU | ||||
| 18 | ILE | ASN | GLY | ARG | ASN | ILE | ARG | LEU | ILE | GLU | ||||
| 19 | ASP | LYS | PRO | ARG | THR | SER | HIS | ARG |
Samples:
sample_1: SRSF6_1-188, [U-13C; U-15N; U-2H], 230 uM; D2O, [U-2H], 7%; potassium phosphate 20 mM; TCEP 2 mM; l-arginine 380 mM; l-glutamate 380 mM; sodium azide 0.02%
sample_2: SRSF6_1-188, [U-99% 13C; U-99% 15N], 330 uM; D2O, [U-2H], 7%; potassium phosphate 20 mM; TCEP 2 mM; l-arginine 380 mM; l-glutamate 380 mM; sodium azide 0.02%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHANH | sample_2 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.6-4.4 - collection, processing
ANALYSIS v2.5.1-2.5.2 - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker AVANCE NEO 900 MHz
- Bruker AVANCE III 950 MHz
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks