BMRB Entry 5308

Title:
Solution Structure and Dynamics of the Human-Escherichia coli Thioredoxin Chimera: Insights into Thermodynamic Stability
Deposition date:
2002-03-02
Original release date:
2002-09-11
Authors:
Dangi, Bindi; Dubrodumov, Anatoliy; Louis, John; Gronenborn, Angela
Citation:

Citation: Dangi, Bindi; Dubrodumov, Anatoliy; Louis, John; Gronenborn, Angela. "Solution Structure and Dynamics of the Human-Escherichia coli Thioredoxin Chimera: Insights into Thermodynamic Stability"  Biochemistry 41, 9376-9388 (2002).
PubMed: 12135359

Assembly members:

Assembly members:
thioredoxin, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts455
15N chemical shifts108
1H chemical shifts678

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1trx-he1

Entities:

Entity 1, trx-he 107 residues - Formula weight is not available

1   METVALLYSGLNILEGLUSERLYSTHRALA
2   PHEGLNGLUALALEUASPALAALAGLYASP
3   LYSLEUVALVALVALASPPHESERALATHR
4   TRPCYSGLYPROCYSLYSMETILELYSPRO
5   PHEPHEHISSERLEUSERGLULYSTYRSER
6   ASNVALILEPHELEUGLUVALASPVALASP
7   ASPALAGLNASPVALALAPROLYSTYRGLY
8   ILEARGGLYILEPROTHRLEULEULEUPHE
9   LYSASNGLYGLUVALALAALATHRLYSVAL
10   GLYALALEUSERLYSGLYGLNLEULYSGLU
11   PHELEUASPALAASNLEUVAL

Samples:

sample_1: thioredoxin, [U-95% 13C; U-95% 15N], 1 – 2 mM

condition_1: pH: 7.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
15N TOCSY-HSQCnot availablenot availablenot available
15N NOESY-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
4D 13C NOESYnot availablenot availablenot available

Software:

NMRPipe - processing

NMR spectrometers:

  • BRUKER DMX 600 MHz
  • BRUKER DMX 500 MHz
  • BRUKER DRX 800 MHz
  • BRUKER DMX 750 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks