BMRB Entry 53039

Name: Homo sapiens UPF2 (1015-1106)
Published: 2025-12-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53039

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Homo sapiens UPF2 (1015-1106)

Deposition date:

2025-04-17

Original release date:

2025-12-08

Authors:

Perez-Borrajero, Cecilia; Hennig, Janosch; Chakrabarti, Sutapa

Citation:

Citation: Perez-Borrajero, Cecilia; Hennig, Janosch; Chakrabarti, Sutapa. "Homo sapiens UPF2 1015-1106" .

Assembly members:

Assembly members:
entity_1, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAASMNDKDSKDSMTEGENL EEDEEEEEGGAETEEQSGNE SEVNEPEEEEGSDNDDDEGE EEEEENTDYLTDSNKENETD EENTEVMIKGGGLKHVP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	182
15N chemical shifts	94
1H chemical shifts	95

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UPF2	1

Entities:

Entity 1, UPF2 97 residues - Formula weight is not available

N-terminal residual tag present (GAASM). The native sequence starts with NDK (residues 1015-1017) and ends with HVP (residues 1104-1106).

1

GLY

ALA

SER

MET

ASN

ASP

LYS

ASP

SER

2

LYS

ASP

SER

MET

THR

GLU

GLY

GLU

ASN

LEU

3

GLU

ASP

GLU

GLY

4

ALA

GLU

THR

GLU

GLN

SER

GLY

ASN

GLU

5

SER

GLU

VAL

ASN

GLU

PRO

GLU

6

GLY

SER

ASP

ASN

ASP

GLU

GLY

GLU

7

GLU

ASN

THR

ASP

TYR

LEU

8

THR

ASP

SER

ASN

LYS

GLU

ASN

GLU

THR

ASP

9

GLU

ASN

THR

GLU

VAL

MET

ILE

LYS

GLY

10

GLY

LEU

LYS

HIS

VAL

PRO

Samples:

sample_1: Homo sapiens UPF2, [U-100% 13C; U-100% 15N], 550 uM; D2O, [U-100% 2H], 10%; sodium azide 0.2%; HEPES 20 mM; sodium chloride 100 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5 - collection

NMRPipe - processing

NMRDraw - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz