BMRB Entry 53013

Name: NMR chemical shift assignment of UEV domain of Ubiquitin-conjugating enzyme E2 variant 3 lactate dehydrogenase (UEVLD)
Published: 2025-11-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53013

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR chemical shift assignment of UEV domain of Ubiquitin-conjugating enzyme E2 variant 3 lactate dehydrogenase (UEVLD)

Deposition date:

2025-04-07

Original release date:

2025-11-14

Authors:

Nyenhuis, David; Vazquez, Jose; Strub, Marie-Paule; Tjandra, Nico

Citation:

Citation: Vazquez, Jose; Nyenhuis, David; Strub, Marie-Paule; Tjandra, Nico. "NMR chemical shift assignment of UEV domain of ubiquitin-conjugating enzyme E2 variant 3 lactate dehydrogenase (UEVLD)" Biomol. NMR Assign. 19, 227-233 (2025).
PubMed: 40571836

Assembly members:

Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMEFDCEGLRRLLGKYKFRD LTVEELRNVNVFFPHFKYSM DTYVFKDSSQKDLLNFTGTI PVMYQGNTYNIPIRFWILDS HPFAPPICFLKPTANMGILV GKHVDAQGRIYLPYLQNWSH PKSVIVGLIKEMIAKFQEEL PMYSLS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	279
15N chemical shifts	135
1H chemical shifts	135

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UEV Domain of UEVLD	1

Entities:

Entity 1, UEV Domain of UEVLD 146 residues - Formula weight is not available

1

GLY

MET

GLU

PHE

ASP

CYS

GLU

GLY

LEU

ARG

2

ARG

LEU

GLY

LYS

TYR

LYS

PHE

ARG

ASP

3

LEU

THR

VAL

GLU

LEU

ARG

ASN

VAL

ASN

4

VAL

PHE

PRO

HIS

PHE

LYS

TYR

SER

MET

5

ASP

THR

TYR

VAL

PHE

LYS

ASP

SER

GLN

6

LYS

ASP

LEU

ASN

PHE

THR

GLY

THR

ILE

7

PRO

VAL

MET

TYR

GLN

GLY

ASN

THR

TYR

ASN

8

ILE

PRO

ILE

ARG

PHE

TRP

ILE

LEU

ASP

SER

9

HIS

PRO

PHE

ALA

PRO

ILE

CYS

PHE

LEU

10

LYS

PRO

THR

ALA

ASN

MET

GLY

ILE

LEU

VAL

11

GLY

LYS

HIS

VAL

ASP

ALA

GLN

GLY

ARG

ILE

12

TYR

LEU

PRO

TYR

LEU

GLN

ASN

TRP

SER

HIS

13

PRO

LYS

SER

VAL

ILE

VAL

GLY

LEU

ILE

LYS

14

GLU

MET

ILE

ALA

LYS

PHE

GLN

GLU

LEU

15

PRO

MET

TYR

SER

LEU

SER

Samples:

sample_1: N-terminal UEV domain of UEVLD, [U-100% 15N], 200 uM; sodium chloride 50 mM; potassium phosphate 20 mM; D2O 6.25%

sample_2: N-terminal UEV domain of UEVLD, [U-100% 15N], 100 uM; sodium chloride 50 mM; potassium phosphate 20 mM; D2O 6.25%

sample_3: N-terminal UEV domain of UEVLD, [U-100% 13C; U-100% 15N], 400 uM; sodium chloride 50 mM; potassium phosphate 20 mM; D2O 6.25%

sample_conditions_1: ionic strength: 50 mM; pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
T2/R2 relaxation	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1

Software:

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz
Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks