BMRB Entry 52984

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52984

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Title:
ADP Ribosylation Factor Arf6 complexed with GDP
Deposition date:
2025-03-28
Original release date:
2025-11-15
Authors:
Hantman, Noam; Koduru, Tejaswi; Peters, Edgar; Jaworek, Michel; McCallum, Scott; Gillilan, Richard; Winter, Roland; Cherfils, Jacqueline; Royer, Catherine
Citation:

Citation: Hantman, Noam; Koduru, Tejaswi; Peters, Edgar; Jaworek, Michel; McCallum, Scott; Gillilan, Richard; Winter, Roland; Cherfils, Jacqueline; Royer, Catherine. "Sequence Determinants of Allosteric Back-to-front Control of the Arf Nucleotide Switch"  J. Mol. Biol. 437, 169361-169361 (2025).
PubMed: 40721149

Assembly members:

Assembly members:
entity_1, polymer, 175 residues, Formula weight is not available
entity_GDP, non-polymer, 443.201 Da.
entity_MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGKVLSKIFGNKEMRILMLG LDAAGKTTILYKLKLGQSVT TIPTVGFNVETVTYKNVKFN VWDVGGQDKIRPLWRHYYTG TQGLIFVVDCADRDRIDEAR QELHRIINDREMRDAIILIF ANKQDLPDAMKPHEIQEKLG LTRIRDRNWYVQPSCATSGD GLYEGLTWLTSNYKS

Data sets:
Data typeCount
13C chemical shifts256
15N chemical shifts140
1H chemical shifts269

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ar61
2GDP2
3Mg3

Entities:

Entity 1, Ar6 175 residues - Formula weight is not available

1   METGLYLYSVALLEUSERLYSILEPHEGLY
2   ASNLYSGLUMETARGILELEUMETLEUGLY
3   LEUASPALAALAGLYLYSTHRTHRILELEU
4   TYRLYSLEULYSLEUGLYGLNSERVALTHR
5   THRILEPROTHRVALGLYPHEASNVALGLU
6   THRVALTHRTYRLYSASNVALLYSPHEASN
7   VALTRPASPVALGLYGLYGLNASPLYSILE
8   ARGPROLEUTRPARGHISTYRTYRTHRGLY
9   THRGLNGLYLEUILEPHEVALVALASPCYS
10   ALAASPARGASPARGILEASPGLUALAARG
11   GLNGLULEUHISARGILEILEASNASPARG
12   GLUMETARGASPALAILEILELEUILEPHE
13   ALAASNLYSGLNASPLEUPROASPALAMET
14   LYSPROHISGLUILEGLNGLULYSLEUGLY
15   LEUTHRARGILEARGASPARGASNTRPTYR
16   VALGLNPROSERCYSALATHRSERGLYASP
17   GLYLEUTYRGLUGLYLEUTHRTRPLEUTHR
18   SERASNTYRLYSSER

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Entity 3, Mg - Mg - 24.305 Da.

1   MG

Samples:

sample_1: Arf6, [U-99% 13C; U-99% 15N], 0.75 mM; GDP 0.75 mM; bis-Tris 50 mM; magnesium chloride 1 mM; sodium chloride 150 mM; DTT 5 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D 15N-separated NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D hC(C)(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D Hc(C)(CO)NH-TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.2 - collection

I-PINE - chemical shift assignment

MONTE v2.2 - chemical shift assignment

NMRPipe - processing

POKY - peak picking

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks