BMRB Entry 52952
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52952
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Payr, Marco; Meyer, Julia; Geissen, Eva-Maria; Duss, Olivier; Hennig, Janosch. "Real-time tracking of mRNP complex assembly reveals multiple mechanisms ensuring efficient translation repression" .
Assembly members:
entity_1, polymer, 186 residues, Formula weight is not available
entity_2, polymer, 10 residues, Formula weight is not available
entity_3, polymer, 72 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24d(+)
Entity Sequences (FASTA):
entity_1: GAASNTNLIVNYLPQDMTDR
ELYALFRAIGPINTCRIMRD
YKTGYSFGYAFVDFTSEMDS
QRAIKVLNGITVRNKRLKVS
YARPGGESIKDTNLYVTNLP
RTITDDQLDTIFGKYGSIVQ
KNILRDKLTGRPRGVAFVRY
NKREEAQEAISALNNVIPEG
GSQPLSVRLAEEHGKAKAAH
FMSQMG
entity_2: UUUUUUUUUU
entity_3: GAMATRETGIIEKLLHSYGF
IQCCERQARLFFHFSQFSGN
IDHLKIGDPVEFEMTYDRRT
GKPIASQVSKIA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 447 |
| 15N chemical shifts | 160 |
| 1H chemical shifts | 160 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sxl dRBD5 | 1 |
| 2 | U10 | 2 |
| 3 | dUnr-CSD1 | 3 |
Entities:
Entity 1, Sxl dRBD5 186 residues - Formula weight is not available
N-terminal GA from purification tag
| 1 | GLY | ALA | ALA | SER | ASN | THR | ASN | LEU | ILE | VAL | ||||
| 2 | ASN | TYR | LEU | PRO | GLN | ASP | MET | THR | ASP | ARG | ||||
| 3 | GLU | LEU | TYR | ALA | LEU | PHE | ARG | ALA | ILE | GLY | ||||
| 4 | PRO | ILE | ASN | THR | CYS | ARG | ILE | MET | ARG | ASP | ||||
| 5 | TYR | LYS | THR | GLY | TYR | SER | PHE | GLY | TYR | ALA | ||||
| 6 | PHE | VAL | ASP | PHE | THR | SER | GLU | MET | ASP | SER | ||||
| 7 | GLN | ARG | ALA | ILE | LYS | VAL | LEU | ASN | GLY | ILE | ||||
| 8 | THR | VAL | ARG | ASN | LYS | ARG | LEU | LYS | VAL | SER | ||||
| 9 | TYR | ALA | ARG | PRO | GLY | GLY | GLU | SER | ILE | LYS | ||||
| 10 | ASP | THR | ASN | LEU | TYR | VAL | THR | ASN | LEU | PRO | ||||
| 11 | ARG | THR | ILE | THR | ASP | ASP | GLN | LEU | ASP | THR | ||||
| 12 | ILE | PHE | GLY | LYS | TYR | GLY | SER | ILE | VAL | GLN | ||||
| 13 | LYS | ASN | ILE | LEU | ARG | ASP | LYS | LEU | THR | GLY | ||||
| 14 | ARG | PRO | ARG | GLY | VAL | ALA | PHE | VAL | ARG | TYR | ||||
| 15 | ASN | LYS | ARG | GLU | GLU | ALA | GLN | GLU | ALA | ILE | ||||
| 16 | SER | ALA | LEU | ASN | ASN | VAL | ILE | PRO | GLU | GLY | ||||
| 17 | GLY | SER | GLN | PRO | LEU | SER | VAL | ARG | LEU | ALA | ||||
| 18 | GLU | GLU | HIS | GLY | LYS | ALA | LYS | ALA | ALA | HIS | ||||
| 19 | PHE | MET | SER | GLN | MET | GLY |
Entity 2, U10 10 residues - Formula weight is not available
| 1 | U | U | U | U | U | U | U | U | U | U |
Entity 3, dUnr-CSD1 72 residues - Formula weight is not available
N-terminal GAMA from purification tag
| 1 | GLY | ALA | MET | ALA | THR | ARG | GLU | THR | GLY | ILE | ||||
| 2 | ILE | GLU | LYS | LEU | LEU | HIS | SER | TYR | GLY | PHE | ||||
| 3 | ILE | GLN | CYS | CYS | GLU | ARG | GLN | ALA | ARG | LEU | ||||
| 4 | PHE | PHE | HIS | PHE | SER | GLN | PHE | SER | GLY | ASN | ||||
| 5 | ILE | ASP | HIS | LEU | LYS | ILE | GLY | ASP | PRO | VAL | ||||
| 6 | GLU | PHE | GLU | MET | THR | TYR | ASP | ARG | ARG | THR | ||||
| 7 | GLY | LYS | PRO | ILE | ALA | SER | GLN | VAL | SER | LYS | ||||
| 8 | ILE | ALA |
Samples:
sample_1: Sxl dRBD5, [U-13C; U-15N; U-2H], 300 uM; U10 RNA 360 uM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 1 mM; dUnr-CSD1, [U-2H], 450 uM
sample_conditions_1: pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRViewJ - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 1000 MHz
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks