BMRB Entry 52906
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52906
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Mohanty, Biswaranjan; Jones, Natasha; Mackay, Joel. "Chemical shift assignments for SET-9/26 PHD domain in isolation
" .
Assembly members:
entity_1, polymer, 92 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: E. coli Vector: pGEX-6P
Entity Sequences (FASTA):
entity_1: GPLGSAAEESWTMRCHCGMD
HGDGDTIECEGCKTWQHMAC
MGLTLKSNTSKYKCEMCLPR
RLPVSKAEAAREQERILNRL
RAAARKQKRKSE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 267 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SET-9/26 PHD domain | 1 |
| 2 | Zn+2, 1 | 2 |
| 3 | Zn+2, 2 | 2 |
Entities:
Entity 1, SET-9/26 PHD domain 92 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | ALA | ALA | GLU | GLU | SER | ||||
| 2 | TRP | THR | MET | ARG | CYS | HIS | CYS | GLY | MET | ASP | ||||
| 3 | HIS | GLY | ASP | GLY | ASP | THR | ILE | GLU | CYS | GLU | ||||
| 4 | GLY | CYS | LYS | THR | TRP | GLN | HIS | MET | ALA | CYS | ||||
| 5 | MET | GLY | LEU | THR | LEU | LYS | SER | ASN | THR | SER | ||||
| 6 | LYS | TYR | LYS | CYS | GLU | MET | CYS | LEU | PRO | ARG | ||||
| 7 | ARG | LEU | PRO | VAL | SER | LYS | ALA | GLU | ALA | ALA | ||||
| 8 | ARG | GLU | GLN | GLU | ARG | ILE | LEU | ASN | ARG | LEU | ||||
| 9 | ARG | ALA | ALA | ALA | ARG | LYS | GLN | LYS | ARG | LYS | ||||
| 10 | SER | GLU |
Entity 2, Zn+2, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: SET-9/26 PHD domain, [U-98% 13C; U-98% 15N], 0.5 mM; sodium phosphate 10 mM; NaCl 50 mM; TCEP.HCl 1 mM
sample_conditions_1: ionic strength: 0.08 M; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 15N-resolved [1H, 1H]-NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 13Cali-resolved [1H, 1H]-NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 13Caro-resolved [1H, 1H]-NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.7 - collection, data analysis
CARA v1.5.5 - chemical shift assignment, data analysis
NMRPipe - Data processing
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks