BMRB Entry 52889
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52889
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Shi, X.; Fedulova, A.S.; Kotova, E.Y.; Maluchenko, N.V.; Armeev, G.A.; Chen, Q.; Prasanna, C.; Sivkina, A.L.; Feofanov, A.V.; Kirpichnikov, M.P.; Nordenskold, L.; Shaytan, A.K.; Studitsky, V.M.. "Histone tetrasome dynamics affects chromatin transcription" Nucleic Acids Res. 53, gkaf356-gkaf356 (2025).
PubMed: 40304183
Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available
entity_2, polymer, 136 residues, Formula weight is not available
entity_3, polymer, 145 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET-3a
Entity Sequences (FASTA):
entity_1: MSGRGKGGKGLGKGGAKRHR
KVLRDNIQGITKPAIRRLAR
RGGVKRISGLIYEETRGVLK
VFLENVIRDAVTYTEHAKRK
TVTAMDVVYALKRQGRTLYG
FGG
entity_2: MARTKQTARKSTGGKAPRKQ
LATKAARKSAPATGGVKKPH
RYRPGTVALREIRRYQKSTE
LLIRKLPFQRLVREIAQDFK
TDLRFQSSAVMALQEACEAY
LVGLFEDTNLCAIHAKRVTI
MPKDIQLARRIRGERA
entity_3: ATCAGAATCCCGGTGCCGAG
GCCGCTCAATTGGTCGTAGA
CAGCTCTAGCACCGCTTAAA
CGCACGTACGCGCTGTCCCC
CGCGTTTTAACCGCCAAGGG
GATTACTCCCTAGTCTCCAG
GCACGTGTCAGATATATACA
TCGAT
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 50 |
| 15N chemical shifts | 39 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human histone H4 | 1 |
| 2 | human histone H3 | 2 |
| 3 | 145 bp DNA | 3 |
Entities:
Entity 1, human histone H4 103 residues - Formula weight is not available
| 1 | MET | SER | GLY | ARG | GLY | LYS | GLY | GLY | LYS | GLY | ||||
| 2 | LEU | GLY | LYS | GLY | GLY | ALA | LYS | ARG | HIS | ARG | ||||
| 3 | LYS | VAL | LEU | ARG | ASP | ASN | ILE | GLN | GLY | ILE | ||||
| 4 | THR | LYS | PRO | ALA | ILE | ARG | ARG | LEU | ALA | ARG | ||||
| 5 | ARG | GLY | GLY | VAL | LYS | ARG | ILE | SER | GLY | LEU | ||||
| 6 | ILE | TYR | GLU | GLU | THR | ARG | GLY | VAL | LEU | LYS | ||||
| 7 | VAL | PHE | LEU | GLU | ASN | VAL | ILE | ARG | ASP | ALA | ||||
| 8 | VAL | THR | TYR | THR | GLU | HIS | ALA | LYS | ARG | LYS | ||||
| 9 | THR | VAL | THR | ALA | MET | ASP | VAL | VAL | TYR | ALA | ||||
| 10 | LEU | LYS | ARG | GLN | GLY | ARG | THR | LEU | TYR | GLY | ||||
| 11 | PHE | GLY | GLY |
Entity 2, human histone H3 136 residues - Formula weight is not available
| 1 | MET | ALA | ARG | THR | LYS | GLN | THR | ALA | ARG | LYS | ||||
| 2 | SER | THR | GLY | GLY | LYS | ALA | PRO | ARG | LYS | GLN | ||||
| 3 | LEU | ALA | THR | LYS | ALA | ALA | ARG | LYS | SER | ALA | ||||
| 4 | PRO | ALA | THR | GLY | GLY | VAL | LYS | LYS | PRO | HIS | ||||
| 5 | ARG | TYR | ARG | PRO | GLY | THR | VAL | ALA | LEU | ARG | ||||
| 6 | GLU | ILE | ARG | ARG | TYR | GLN | LYS | SER | THR | GLU | ||||
| 7 | LEU | LEU | ILE | ARG | LYS | LEU | PRO | PHE | GLN | ARG | ||||
| 8 | LEU | VAL | ARG | GLU | ILE | ALA | GLN | ASP | PHE | LYS | ||||
| 9 | THR | ASP | LEU | ARG | PHE | GLN | SER | SER | ALA | VAL | ||||
| 10 | MET | ALA | LEU | GLN | GLU | ALA | CYS | GLU | ALA | TYR | ||||
| 11 | LEU | VAL | GLY | LEU | PHE | GLU | ASP | THR | ASN | LEU | ||||
| 12 | CYS | ALA | ILE | HIS | ALA | LYS | ARG | VAL | THR | ILE | ||||
| 13 | MET | PRO | LYS | ASP | ILE | GLN | LEU | ALA | ARG | ARG | ||||
| 14 | ILE | ARG | GLY | GLU | ARG | ALA |
Entity 3, 145 bp DNA 145 residues - Formula weight is not available
| 1 | DA | DT | DC | DA | DG | DA | DA | DT | DC | DC | ||||
| 2 | DC | DG | DG | DT | DG | DC | DC | DG | DA | DG | ||||
| 3 | DG | DC | DC | DG | DC | DT | DC | DA | DA | DT | ||||
| 4 | DT | DG | DG | DT | DC | DG | DT | DA | DG | DA | ||||
| 5 | DC | DA | DG | DC | DT | DC | DT | DA | DG | DC | ||||
| 6 | DA | DC | DC | DG | DC | DT | DT | DA | DA | DA | ||||
| 7 | DC | DG | DC | DA | DC | DG | DT | DA | DC | DG | ||||
| 8 | DC | DG | DC | DT | DG | DT | DC | DC | DC | DC | ||||
| 9 | DC | DG | DC | DG | DT | DT | DT | DT | DA | DA | ||||
| 10 | DC | DC | DG | DC | DC | DA | DA | DG | DG | DG | ||||
| 11 | DG | DA | DT | DT | DA | DC | DT | DC | DC | DC | ||||
| 12 | DT | DA | DG | DT | DC | DT | DC | DC | DA | DG | ||||
| 13 | DG | DC | DA | DC | DG | DT | DG | DT | DC | DA | ||||
| 14 | DG | DA | DT | DA | DT | DA | DT | DA | DC | DA | ||||
| 15 | DT | DC | DG | DA | DT |
Samples:
sample_1: Histone H4, [U-98% 13C; U-98% 15N], 5 % w/w; Histone H3 6.4 % w/w; 145 bp DNA 18.6 % w/w; H2O 70 % w/w
sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 12 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NCA | sample_1 | isotropic | sample_conditions_1 |
| 2D NCO | sample_1 | isotropic | sample_conditions_1 |
| 2D DARR | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - data analysis
NMR spectrometers:
- Bruker AVANCE III 800 MHz