BMRB Entry 52865

Name: Backbone 1H, 13C, 15N chemical shifts of ICL3 from M1 muscarinic cholinergic receptor [Homo sapiens]
Published: 2025-11-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52865

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N chemical shifts of ICL3 from M1 muscarinic cholinergic receptor [Homo sapiens]

Deposition date:

2025-01-16

Original release date:

2025-11-18

Authors:

Wang, Jiannan; Niu, Xiaogang; Jin, Changwen; Hu, Yunfei

Citation:

Citation: Wang, Jiannan; Niu, Xiaogang; Jin, Changwen; Hu, Yunfei. "1H, 13C and 15N resonance assignments of the third intracellular loop of the muscarinic acetylcholine receptor M1" Biomol. NMR Assign. 19, 147-151 (2025).
PubMed: 40178795

Assembly members:

Assembly members:
entity_1, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QGSETPGKGGGSSSSSERSQ PGAEGSPETPPGRCCRCCRA PRLLQAYSWKEEEEEDEGSM ESLTSSEGEEPGSEVVIKMP MVDPEAQAPTKQPPRSSPNT VKRPTKKGRDRAGKGQKPRG KEQLAKRK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	209
15N chemical shifts	100
1H chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ICL3 from M1 muscarinic cholinergic receptor [Homo sapiens]	1

Entities:

Entity 1, ICL3 from M1 muscarinic cholinergic receptor [Homo sapiens] 128 residues - Formula weight is not available

1

GLN

GLY

SER

GLU

THR

PRO

GLY

LYS

GLY

2

GLY

SER

GLU

ARG

SER

GLN

3

PRO

GLY

ALA

GLU

GLY

SER

PRO

GLU

THR

PRO

4

PRO

GLY

ARG

CYS

ARG

CYS

ARG

ALA

5

PRO

ARG

LEU

GLN

ALA

TYR

SER

TRP

LYS

6

GLU

ASP

GLU

GLY

SER

MET

7

GLU

SER

LEU

THR

SER

GLU

GLY

GLU

8

PRO

GLY

SER

GLU

VAL

ILE

LYS

MET

PRO

9

MET

VAL

ASP

PRO

GLU

ALA

GLN

ALA

PRO

THR

10

LYS

GLN

PRO

ARG

SER

PRO

ASN

THR

11

VAL

LYS

ARG

PRO

THR

LYS

GLY

ARG

ASP

12

ARG

ALA

GLY

LYS

GLY

GLN

LYS

PRO

ARG

GLY

13

LYS

GLU

GLN

LEU

ALA

LYS

ARG

LYS

Samples:

sample_1: ICL3 from M1 muscarinic cholinergic receptor [Homo sapiens], [U-100% 13C; U-100% 15N], 0.5 mM; HEPES 20 mM; NaCl 0.1 M; DTT 5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)N	sample_1	isotropic	sample_conditions_1
3D HN(COCA)N	sample_1	isotropic	sample_conditions_1
3D HN(CA)NH	sample_1	isotropic	sample_conditions_1
3D HN(COCA)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - data analysis

NMR spectrometers:

Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks