BMRB Entry 52795

Name: Phosphorylated Drosophila RNA polymerase II Rpb1 CTD 1657-1739 (Dyr1ka, 8P)
Published: 2025-07-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52795

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Phosphorylated Drosophila RNA polymerase II Rpb1 CTD 1657-1739 (Dyr1ka, 8P)

Deposition date:

2025-01-08

Original release date:

2025-07-03

Authors:

Chen, Wei; Showalter, Scott

Citation:

Citation: Chen, Wei; Laremore, Tatiana; Yennawar, Neela; Showalter, Scott. "Phosphorylation patterns modulate the transient secondary structure of RNA polymerase II CTD without altering its global conformation" .

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His6 MBP TEV LIC cloning vector (1M)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SNAGFAGSGSNIYSPGNAYS PSSSNYSPNSPSYSPTSPSY SPSSPSYSPTSPCYSPTSPS YSPTSPNYTPVTPSYSPTSP NYSASPQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	256
15N chemical shifts	86

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CTD2	1

Entities:

Entity 1, CTD2 87 residues - Formula weight is not available

Residues 1-4 (amino acids SNAG) are cloning artifacts remaining after MBP cleavage by TEV.

1

SER

ASN

ALA

GLY

PHE

ALA

GLY

SER

GLY

SER

2

ASN

ILE

TYR

SER

PRO

GLY

ASN

ALA

TYR

SER

3

PRO

SER

ASN

TYR

SER

PRO

ASN

SER

4

PRO

SER

TYR

SER

PRO

THR

SER

PRO

SER

TYR

5

SER

PRO

SER

PRO

SER

TYR

SER

PRO

THR

6

SER

PRO

CYS

TYR

SER

PRO

THR

SER

PRO

SER

7

TYR

SER

PRO

THR

SER

PRO

ASN

TYR

THR

PRO

8

VAL

THR

PRO

SER

TYR

SER

PRO

THR

SER

PRO

9

ASN

TYR

SER

ALA

SER

PRO

GLN

Samples:

sample_1: Drosophila Rpb1 1657-1739, [U-100% 13C; U-100% 15N], 0.8 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-15N CON-IPAP	sample_1	isotropic	sample_conditions_1
3D (H)CCCON-IPAP	sample_1	isotropic	sample_conditions_1
3D (HACA)N(CA)CON-IPAP	sample_1	isotropic	sample_conditions_1
3D (HACA)N(CA)NCO-IPAP	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz