BMRB Entry 52728

Name: Backbone Chemical Shift Assignments of CaV1.4 PCRD
Published: 2025-11-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52728

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Chemical Shift Assignments of CaV1.4 PCRD

Deposition date:

2024-11-27

Original release date:

2025-11-20

Authors:

Ahoulou, Effibe; Yadav, Deepak; Anderson, David; Ames, James

Citation:

Citation: Yadav, Deepak Kumar; Ahoulou, Effibe; Anderson, David; Bej, Aritra; Hell, Johannes; Ames, James. "L-Type Voltage-Gated Ca2+ Channel C-Terminal Proximal and Distal Domains (PCRD and DCRD) Bind to the IQ-Motif and May Modulate Channel Function" Biochemistry 64, 1933-1942 (2025).
PubMed: 40238689

Assembly members:

Assembly members:
entity_1, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMA507

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ALQAGLRSLQDLGPEMRQAL TCDTEEEEEEGQEGVEEEDE KDLETNKATMVSQPSARRGS GIS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	122
15N chemical shifts	35
1H chemical shifts	137

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PCRD	1

Entities:

Entity 1, PCRD 63 residues - Formula weight is not available

The sequence corresponds to the CaV1.4 proximal C-terminal regulatory domain (called PCRD).

1

ALA

LEU

GLN

ALA

GLY

LEU

ARG

SER

LEU

GLN

2

ASP

LEU

GLY

PRO

GLU

MET

ARG

GLN

ALA

LEU

3

THR

CYS

ASP

THR

GLU

4

GLY

GLN

GLU

GLY

VAL

GLU

ASP

GLU

5

LYS

ASP

LEU

GLU

THR

ASN

LYS

ALA

THR

MET

6

VAL

SER

GLN

PRO

SER

ALA

ARG

GLY

SER

7

GLY

ILE

SER

Samples:

sample_1: PCRD, [U-95% 15N], 0.5 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks