BMRB Entry 52720
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52720
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Qu, Xiaozhan; Wang, Simin; Zhao, Shuo; Wan, Chanjuan; Xu, Weiya; Huang, Chengdong. "The dynamic triage interplay of Hsp90 with its chaperone cycle and client binding" Nat. Commun. 15, 10661-10661 (2024).
PubMed: 39663352
Assembly members:
entity_1, polymer, 266 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-16a
Entity Sequences (FASTA):
entity_1: AQALWTRNKSEITDEEYKEF
YKHIAHDFNDPLTWSHNRVE
GKQEYTSLLYIPSQAPWDMW
NRDHKHGLKLYVQRVFIMDD
AEQFMPNYLRFVRGLIDSSD
LPLNVSREILQDSTVTRNLR
NALTKRVLQMLEKLAKDDAE
KYQTFWQQFGLVLKEGPAED
FANQEAIAKLLRFASTHTDS
SAQTVSLEDYVSRMKEGQEK
IYYITADSYAAAKSSPHLEL
LRKKGIEVLLLSDRIDEWMM
NYLTEFDGKPFQSVSKVDES
LEKLAD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 603 |
| 15N chemical shifts | 227 |
| 1H chemical shifts | 533 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | The NTD of E coli Hsp90 | 1 |
Entities:
Entity 1, The NTD of E coli Hsp90 266 residues - Formula weight is not available
| 1 | ALA | GLN | ALA | LEU | TRP | THR | ARG | ASN | LYS | SER | ||||
| 2 | GLU | ILE | THR | ASP | GLU | GLU | TYR | LYS | GLU | PHE | ||||
| 3 | TYR | LYS | HIS | ILE | ALA | HIS | ASP | PHE | ASN | ASP | ||||
| 4 | PRO | LEU | THR | TRP | SER | HIS | ASN | ARG | VAL | GLU | ||||
| 5 | GLY | LYS | GLN | GLU | TYR | THR | SER | LEU | LEU | TYR | ||||
| 6 | ILE | PRO | SER | GLN | ALA | PRO | TRP | ASP | MET | TRP | ||||
| 7 | ASN | ARG | ASP | HIS | LYS | HIS | GLY | LEU | LYS | LEU | ||||
| 8 | TYR | VAL | GLN | ARG | VAL | PHE | ILE | MET | ASP | ASP | ||||
| 9 | ALA | GLU | GLN | PHE | MET | PRO | ASN | TYR | LEU | ARG | ||||
| 10 | PHE | VAL | ARG | GLY | LEU | ILE | ASP | SER | SER | ASP | ||||
| 11 | LEU | PRO | LEU | ASN | VAL | SER | ARG | GLU | ILE | LEU | ||||
| 12 | GLN | ASP | SER | THR | VAL | THR | ARG | ASN | LEU | ARG | ||||
| 13 | ASN | ALA | LEU | THR | LYS | ARG | VAL | LEU | GLN | MET | ||||
| 14 | LEU | GLU | LYS | LEU | ALA | LYS | ASP | ASP | ALA | GLU | ||||
| 15 | LYS | TYR | GLN | THR | PHE | TRP | GLN | GLN | PHE | GLY | ||||
| 16 | LEU | VAL | LEU | LYS | GLU | GLY | PRO | ALA | GLU | ASP | ||||
| 17 | PHE | ALA | ASN | GLN | GLU | ALA | ILE | ALA | LYS | LEU | ||||
| 18 | LEU | ARG | PHE | ALA | SER | THR | HIS | THR | ASP | SER | ||||
| 19 | SER | ALA | GLN | THR | VAL | SER | LEU | GLU | ASP | TYR | ||||
| 20 | VAL | SER | ARG | MET | LYS | GLU | GLY | GLN | GLU | LYS | ||||
| 21 | ILE | TYR | TYR | ILE | THR | ALA | ASP | SER | TYR | ALA | ||||
| 22 | ALA | ALA | LYS | SER | SER | PRO | HIS | LEU | GLU | LEU | ||||
| 23 | LEU | ARG | LYS | LYS | GLY | ILE | GLU | VAL | LEU | LEU | ||||
| 24 | LEU | SER | ASP | ARG | ILE | ASP | GLU | TRP | MET | MET | ||||
| 25 | ASN | TYR | LEU | THR | GLU | PHE | ASP | GLY | LYS | PRO | ||||
| 26 | PHE | GLN | SER | VAL | SER | LYS | VAL | ASP | GLU | SER | ||||
| 27 | LEU | GLU | LYS | LEU | ALA | ASP |
Samples:
sample_1: The MD of E coli Hsp90, [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3], 500 uM; Phosphate 50 mM; NaCl 100 mM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - collection
NMRViewJ - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz