BMRB Entry 52714

Name: 1H, 13C, 15N and 31P chemical shift assignment of the first stemp-loop Guanidine-II riboswitch from Escherichia coli
Published: 2025-11-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52714

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C, 15N and 31P chemical shift assignment of the first stemp-loop Guanidine-II riboswitch from Escherichia coli

Deposition date:

2024-11-22

Original release date:

2025-11-20

Authors:

Koob, Tatjana; Doepp, Silas; Schwalbe, Harald

Citation:

Citation: Koob, Tatjana; Doepp, Silas; Schwalbe, Harald. "1H, 13C, 15N and 31P chemical shift assignment of the first stem-loop Guanidine-II riboswitch from Escherichia coli" Biomol. NMR Assignments 19, 53-58 (2025).
PubMed: 39890743

Assembly members:

Assembly members:
entity_1, polymer, 23 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pUC57

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGUUUGCAGGACGACCUGCA AAC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	169
15N chemical shifts	53
1H chemical shifts	195
31P chemical shifts	23

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Gdn23	1

Entities:

Entity 1, Gdn23 23 residues - Formula weight is not available

1

G

U

G

C

A

G

2

A

C

G

A

C

U

G

C

A

3

A

C

Samples:

sample_1: Gdn23 660 uM; potassium chloride 50 mM; potassium phosphate 25 mM; DSS 7.5 nM

sample_2: Gdn23, [U-100% 13C; U-100% 15N], 700 uM; potassium chloride 50 mM; potassium phosphate 25 mM; DSS 7.5 nM

sample_3: Gdn23, [U-100% 13C; U-100% 15N], 700 uM; potassium chloride 50 mM; potassium phosphate 25 mM; DSS 7.5 nM

sample_conditions_1: ionic strength: 75 mM; pH: 6.2; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_2	isotropic	sample_conditions_1
2D 1H-15N CPMG NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC NH2 only	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C H(N)CO	sample_2	isotropic	sample_conditions_1
3D NOESY HSQC	sample_2	isotropic	sample_conditions_1
4D CC NOESY	sample_2	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D H(C)P-CCH-TOCSY	sample_3	isotropic	sample_conditions_1

Software:

TOPSPIN - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE NEO 600 MHz
Bruker AVANCE III 700 MHz
Bruker AVANCE III 800 MHz
Bruker AVANCE NEO 900 MHz