BMRB Entry 52702

Name: Backbone Chemical Shift Assignments of CaV1.2 PCRD with S1700D
Published: 2025-11-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52702

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Chemical Shift Assignments of CaV1.2 PCRD with S1700D

Deposition date:

2024-11-15

Original release date:

2025-11-20

Authors:

Ahoulou, Effibe; Yadav, Deepak; Anderson, David; Ames, James

Citation:

Citation: Yadav, Deepak Kumar; Ahoulou, Effibe; Anderson, David; Bej, Aritra; Hell, Johannes; Ames, James. "L-Type Voltage-Gated Ca2+ Channel C-Terminal Proximal and Distal Domains (PCRD and DCRD) Bind to the IQ-Motif and May Modulate Channel Function" Biochemistry 64, 1933-1942 (2025).
PubMed: 40238689

Assembly members:

Assembly members:
entity_1, polymer, 71 residues, 10074 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMA507

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SLQAGLRTLHDIGPEIRRAI DGDLTAEEELDKAMKEAVSA ASEDDIFRRAGGLFGNHVSY YQSDSRSNFPQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	151
15N chemical shifts	43
1H chemical shifts	169

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PCRD	1

Entities:

Entity 1, PCRD 71 residues - 10074 Da.

The sequence corresponds to the CaV1.2 proximal C-terminal regulatory domain (called PCRD) with S1700D mutation.

1

SER

LEU

GLN

ALA

GLY

LEU

ARG

THR

LEU

HIS

2

ASP

ILE

GLY

PRO

GLU

ILE

ARG

ALA

ILE

3

ASP

GLY

ASP

LEU

THR

ALA

GLU

LEU

4

ASP

LYS

ALA

MET

LYS

GLU

ALA

VAL

SER

ALA

5

ALA

SER

GLU

ASP

ILE

PHE

ARG

ALA

6

GLY

LEU

PHE

GLY

ASN

HIS

VAL

SER

TYR

7

TYR

GLN

SER

ASP

SER

ARG

SER

ASN

PHE

PRO

8

GLN

Samples:

sample_1: PCRD, [U-95% 15N], 0.5 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks