BMRB Entry 52696

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52696

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Title:
Tau 151-391 WT
Deposition date:
2024-11-13
Original release date:
2026-05-13
Authors:
Lovestam, Sofia; Wagstaff, Jane; Freund, Stefan; Scheres, Sjors
Citation:

Citation: Lovestam, Sofia; Wagstaff, Jane; Katinelos, Taxiarchis; Freund, Stefan; Goedert, Michel; Scheres, Sjors. "Twelve phoshomimetic mutations induce the assembly of recombinant full-length human tau into paired helical filaments"  elife ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 241 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRK172

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: IATPRGAAPPGQKGQANATR IPAKTPPAPKTPPSSGEPPK SGDRSGYSSPGSPGTPGSRS RTPSLPTPPTREPKKVAVVR TPPKSPSSAKSRLQTAPVPM PDLKNVKSKIGSTENLKHQP GGGKVQIINKKLDLSNVQSK CGSKDNIKHVPGGGSVQIVY KPVDLSKVTSKCGSLGNIHH KPGGGQVEVKSEKLDFKDRV QSKIGSLDNITHVPGGGNKK IETHKLTFRENAKAKTDHGA E

Data sets:
Data typeCount
13C chemical shifts597
15N chemical shifts208
1H chemical shifts181

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tau 151-391 WT1

Entities:

Entity 1, Tau 151-391 WT 241 residues - Formula weight is not available

1   ILEALATHRPROARGGLYALAALAPROPRO
2   GLYGLNLYSGLYGLNALAASNALATHRARG
3   ILEPROALALYSTHRPROPROALAPROLYS
4   THRPROPROSERSERGLYGLUPROPROLYS
5   SERGLYASPARGSERGLYTYRSERSERPRO
6   GLYSERPROGLYTHRPROGLYSERARGSER
7   ARGTHRPROSERLEUPROTHRPROPROTHR
8   ARGGLUPROLYSLYSVALALAVALVALARG
9   THRPROPROLYSSERPROSERSERALALYS
10   SERARGLEUGLNTHRALAPROVALPROMET
11   PROASPLEULYSASNVALLYSSERLYSILE
12   GLYSERTHRGLUASNLEULYSHISGLNPRO
13   GLYGLYGLYLYSVALGLNILEILEASNLYS
14   LYSLEUASPLEUSERASNVALGLNSERLYS
15   CYSGLYSERLYSASPASNILELYSHISVAL
16   PROGLYGLYGLYSERVALGLNILEVALTYR
17   LYSPROVALASPLEUSERLYSVALTHRSER
18   LYSCYSGLYSERLEUGLYASNILEHISHIS
19   LYSPROGLYGLYGLYGLNVALGLUVALLYS
20   SERGLULYSLEUASPPHELYSASPARGVAL
21   GLNSERLYSILEGLYSERLEUASPASNILE
22   THRHISVALPROGLYGLYGLYASNLYSLYS
23   ILEGLUTHRHISLYSLEUTHRPHEARGGLU
24   ASNALALYSALALYSTHRASPHISGLYALA
25   GLU

Samples:

sample_1: Tau 297-391 WT, [U-98% 13C; U-98% 15N], 300 uM; D2O, [U-99% 2H], 5%; potassium phosphate 50 mM; sodium chloride 150 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOCANNHsample_1isotropicsample_conditions_1
3D C_HCACONsample_1isotropicsample_conditions_1
3D C_HCANCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6.0 - collection

NMRFAM-SPARKY - peak picking

NMRFx Processor - processing

MARS - data analysis

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks