Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52694

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Tau297-441 wt
Published: 2026-05-13

Title:

Tau297-441 wt

Deposition date:

2024-11-13

Original release date:

2026-05-13

Authors:

Lovestam, Sofia; Wagstaff, Jane; Freund, Stefan; Scheres, Sjors

Citation:

Citation: Lovestam, Sofia; Wagstaff, Jane; Katinelos, Taxiarchis; Freund, Stefan; Goedert, Michel; Scheres, Sjors. "Twelve phoshomimetic mutations induce the assembly of recombinant full-length human tau into paired helical filaments" elife ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRK172

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: IKHVPGGGSVQIVYKPVDLS KVTSKCGSLGNIHHKPGGGQ VEVKSEKLDFKDRVQSKIGS LDNITHVPGGGNKKIETHKL TFRENAKAKTDHGAEIVYKS PVVSGDTSPRHLSNVSSTGS IDMVDSPQLATLADEVSASL AKQGL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	406
15N chemical shifts	138
1H chemical shifts	131

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tau 297-441 WT	1

Entities:

Entity 1, Tau 297-441 WT 145 residues - Formula weight is not available

1

ILE

LYS

HIS

VAL

PRO

GLY

SER

VAL

2

GLN

ILE

VAL

TYR

LYS

PRO

VAL

ASP

LEU

SER

3

LYS

VAL

THR

SER

LYS

CYS

GLY

SER

LEU

GLY

4

ASN

ILE

HIS

LYS

PRO

GLY

GLN

5

VAL

GLU

VAL

LYS

SER

GLU

LYS

LEU

ASP

PHE

6

LYS

ASP

ARG

VAL

GLN

SER

LYS

ILE

GLY

SER

7

LEU

ASP

ASN

ILE

THR

HIS

VAL

PRO

GLY

8

GLY

ASN

LYS

ILE

GLU

THR

HIS

LYS

LEU

9

THR

PHE

ARG

GLU

ASN

ALA

LYS

ALA

LYS

THR

10

ASP

HIS

GLY

ALA

GLU

ILE

VAL

TYR

LYS

SER

11

PRO

VAL

SER

GLY

ASP

THR

SER

PRO

ARG

12

HIS

LEU

SER

ASN

VAL

SER

THR

GLY

SER

13

ILE

ASP

MET

VAL

ASP

SER

PRO

GLN

LEU

ALA

14

THR

LEU

ALA

ASP

GLU

VAL

SER

ALA

SER

LEU

15

ALA

LYS

GLN

GLY

LEU

Samples:

sample_1: Tau 297-441 WT, [U-98% 13C; U-98% 15N], 300 uM; D2O, [U-99% 2H], 5%; potassium phosphate 50 mM; sodium chloride 150 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCOCANNH	sample_1	isotropic	sample_conditions_1
3D C_HCACON	sample_1	isotropic	sample_conditions_1
3D C_HCANCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.6.0 - collection

NMRFAM-SPARKY - peak picking

MddNMR - processing

MARS - data analysis

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 52694