BMRB Entry 52651

Name: Backbone Chemical Shift Assignments of CaV1.2 DCRD
Published: 2025-11-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52651

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Chemical Shift Assignments of CaV1.2 DCRD

Deposition date:

2024-10-22

Original release date:

2025-11-20

Authors:

Ahoulou, Effibe; Yadav, Deepak; Anderson, David; Ames, James

Citation:

Citation: Yadav, Deepak Kumar; Ahoulou, Effibe; Anderson, David; Bej, Aritra; Hell, Johannes; Ames, James. "L-Type Voltage-Gated Ca2+ Channel C-Terminal Proximal and Distal Domains (PCRD and DCRD) Bind to the IQ-Motif and May Modulate Channel Function" Biochemistry 64, 1933-1942 (2025).
PubMed: 40238689

Assembly members:

Assembly members:
entity_1, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMA507

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSASSLVEAVLISEGLGQFA QDPNFIEVTTQELADACDMT IEEMENAADNILSGG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	149
15N chemical shifts	47
1H chemical shifts	171

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DCRD	1

Entities:

Entity 1, DCRD 55 residues - Formula weight is not available

The sequence corresponds to the CaV1.2 distal C-terminal regulatory domain (called DCRD).

1

GLY

SER

ALA

SER

LEU

VAL

GLU

ALA

VAL

2

LEU

ILE

SER

GLU

GLY

LEU

GLY

GLN

PHE

ALA

3

GLN

ASP

PRO

ASN

PHE

ILE

GLU

VAL

THR

4

GLN

GLU

LEU

ALA

ASP

ALA

CYS

ASP

MET

THR

5

ILE

GLU

MET

GLU

ASN

ALA

ASP

ASN

6

ILE

LEU

SER

GLY

Samples:

sample_1: DCRD, [U-95% 15N], 0.5 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks