BMRB Entry 52650

Name: Backbone assignment for yeast Sla1 SH3 domains 1 and 2
Published: 2024-10-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52650

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment for yeast Sla1 SH3 domains 1 and 2

Deposition date:

2024-10-22

Original release date:

2024-10-28

Authors:

Williamson, Mike; Rowe, Michelle; Ayscough, Kathryn

Citation:

Citation: Hancock, Lewis; Palmer, John; Allwood, Ellen; Smaczynska-de Rooij, Iwona; Rowe, Michelle; Williamson, Mike; Ayscough, Kathryn. "Initiation and Regulation of Actin Nucleation by Competitive Binding of SH3 domains and Actin at Proline-rich regions on Yeast Las17/WASp" .

Assembly members:

Assembly members:
entity_1, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSLGIYRAVYAYEPQTP EELAIQEDDLLYLLQKSDID DWWTVKKRVIGSDSEEPVGL VPSTYIEEAPVLKKVRAIYD YEQVQNADEELTFHENDVFD VFDDKDADWLLVKSTVSNEF GFIPGNYVEPEN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	387
15N chemical shifts	123
1H chemical shifts	123

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sla1	1

Entities:

Entity 1, Sla1 132 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

LEU

GLY

ILE

TYR

ARG

2

ALA

VAL

TYR

ALA

TYR

GLU

PRO

GLN

THR

PRO

3

GLU

LEU

ALA

ILE

GLN

GLU

ASP

LEU

4

LEU

TYR

LEU

GLN

LYS

SER

ASP

ILE

ASP

5

ASP

TRP

THR

VAL

LYS

ARG

VAL

ILE

6

GLY

SER

ASP

SER

GLU

PRO

VAL

GLY

LEU

7

VAL

PRO

SER

THR

TYR

ILE

GLU

ALA

PRO

8

VAL

LEU

LYS

VAL

ARG

ALA

ILE

TYR

ASP

9

TYR

GLU

GLN

VAL

GLN

ASN

ALA

ASP

GLU

10

LEU

THR

PHE

HIS

GLU

ASN

ASP

VAL

PHE

ASP

11

VAL

PHE

ASP

LYS

ASP

ALA

ASP

TRP

LEU

12

LEU

VAL

LYS

SER

THR

VAL

SER

ASN

GLU

PHE

13

GLY

PHE

ILE

PRO

GLY

ASN

TYR

VAL

GLU

PRO

14

GLU

ASN

Samples:

sample_1: Sla1, [U-15N; U-13C], 300 uM; DSS 100 uM; TRIS 50 mM; sodium phosphate 50 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1 - collection

NMR spectrometers:

Bruker Avance 800 MHz