BMRB Entry 52639

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52639

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Title:
Backbone chemical-shift assignment of the N-terminal domain of the GerI germinant receptor A subunit from Bacillus cereus
Deposition date:
2024-10-11
Original release date:
2025-01-20
Authors:
Pustovalova, Yulia; Li, Yunfeng; Hoch, Jeffrey; Hao, Bing
Citation:

Citation: Pustovalova, Yulia; Li, Yunfeng; Hoch, Jeffrey; Hao, Bing. "Backbone assignment of the N-terminal domain of the A subunit of the Bacillus cereus GerI germinant receptor"  Biomol. NMR Assignments ., .-. (2025).
PubMed: 39826051

Assembly members:

Assembly members:
entity_1, polymer, 251 residues, 28661 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: Bacillus cereus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGGRDFTKPEKDRIHSLQNL IEKLKKSSDFVNYHTSDDET MPYWISYYRPSLDGEKLQKY LMPTLLERPNASLEELKEHI PMSGITITNDLQKIEDMVLK GHAIIQLNQQDQKCMLANIA IDNYRAPTPPLNESTVIGPQ EGFVEDIDTNINLVRKRLPV LDLQTKEMIIGEFSKTKVVM MYLDNLAEKDNVDFLEESLR ALEYDQINDSAYLQELMGEK SIFPLYINTERTDRVTKALI DGKIAIFVDGS

Data sets:
Data typeCount
13C chemical shifts594
15N chemical shifts203
1H chemical shifts203

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GerIA-N1

Entities:

Entity 1, GerIA-N 251 residues - 28661 Da.

Residues 1-4 are artificial from cloning. Residues 5-251 represent 238-484 segment of GerIA corresponding to its N-terminal domain (GerIA-N).

1   GLYGLYGLYARGASPPHETHRLYSPROGLU
2   LYSASPARGILEHISSERLEUGLNASNLEU
3   ILEGLULYSLEULYSLYSSERSERASPPHE
4   VALASNTYRHISTHRSERASPASPGLUTHR
5   METPROTYRTRPILESERTYRTYRARGPRO
6   SERLEUASPGLYGLULYSLEUGLNLYSTYR
7   LEUMETPROTHRLEULEUGLUARGPROASN
8   ALASERLEUGLUGLULEULYSGLUHISILE
9   PROMETSERGLYILETHRILETHRASNASP
10   LEUGLNLYSILEGLUASPMETVALLEULYS
11   GLYHISALAILEILEGLNLEUASNGLNGLN
12   ASPGLNLYSCYSMETLEUALAASNILEALA
13   ILEASPASNTYRARGALAPROTHRPROPRO
14   LEUASNGLUSERTHRVALILEGLYPROGLN
15   GLUGLYPHEVALGLUASPILEASPTHRASN
16   ILEASNLEUVALARGLYSARGLEUPROVAL
17   LEUASPLEUGLNTHRLYSGLUMETILEILE
18   GLYGLUPHESERLYSTHRLYSVALVALMET
19   METTYRLEUASPASNLEUALAGLULYSASP
20   ASNVALASPPHELEUGLUGLUSERLEUARG
21   ALALEUGLUTYRASPGLNILEASNASPSER
22   ALATYRLEUGLNGLULEUMETGLYGLULYS
23   SERILEPHEPROLEUTYRILEASNTHRGLU
24   ARGTHRASPARGVALTHRLYSALALEUILE
25   ASPGLYLYSILEALAILEPHEVALASPGLY
26   SER

Samples:

sample_1: GerIA-N, [U-100% 13C; U-100% 15N; U-80% 2H], 0.45 mM; HEPES 20 mM; sodium chloride 50 mM; beta-mercaptoethanol 2 mM; sodium azide 0.01%

sample_2: GerIA-N, [U-100% 15N], 0.45 mM; HEPES 20 mM; sodium chloride 50 mM; beta-mercaptoethanol 2 mM; sodium azide 0.01%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe - processing

CARA - chemical shift assignment

VNMR - collection

TALOS+ - data analysis

NMR spectrometers:

  • Agilent INOVA 800 MHz
  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks