BMRB Entry 52617

Name: Backbone chemical shift assignments of DRB2 dsRBD2 (i.e., DRB2 (84-196))
Published: 2025-11-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52617

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone chemical shift assignments of DRB2 dsRBD2 (i.e., DRB2 (84-196))

Deposition date:

2024-09-24

Original release date:

2025-11-19

Authors:

Patra, Debadutta; Rai, Upasana; Deshmukh, Mandar V.

Citation:

Citation: Patra, Debadutta; Rai, Upasana; Deshmukh, Mandar V.. "Chemical shift assignments of DRB2 domains, a dsRNA binding protein in A. thaliana RNAi pathway" Biomol. NMR Assignments 19, 71-76 (2025).
PubMed: 39881054

Assembly members:

Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: METGVYKNLLQEIAQRVGAP LPRYTTFRSGLGHQPVFTGT VELAGITFTGDPAKNKKQAE KNAAMAAWSSLKQLAKETSS SMPEPENIDELEQVIIARAL INYRIKENIGTGSSLEHHHH HH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	304
15N chemical shifts	94
1H chemical shifts	94

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DRB2 dsRBD2	1

Entities:

Entity 1, DRB2 dsRBD2 122 residues - Formula weight is not available

1

MET

GLU

THR

GLY

VAL

TYR

LYS

ASN

LEU

2

GLN

GLU

ILE

ALA

GLN

ARG

VAL

GLY

ALA

PRO

3

LEU

PRO

ARG

TYR

THR

PHE

ARG

SER

GLY

4

LEU

GLY

HIS

GLN

PRO

VAL

PHE

THR

GLY

THR

5

VAL

GLU

LEU

ALA

GLY

ILE

THR

PHE

THR

GLY

6

ASP

PRO

ALA

LYS

ASN

LYS

GLN

ALA

GLU

7

LYS

ASN

ALA

MET

ALA

TRP

SER

8

LEU

LYS

GLN

LEU

ALA

LYS

GLU

THR

SER

9

SER

MET

PRO

GLU

PRO

GLU

ASN

ILE

ASP

GLU

10

LEU

GLU

GLN

VAL

ILE

ALA

ARG

ALA

LEU

11

ILE

ASN

TYR

ARG

ILE

LYS

GLU

ASN

ILE

GLY

12

THR

GLY

SER

LEU

GLU

HIS

13

HIS

Samples:

sample_1: DRB2 dsRBD2, [U-98% 15N], 200 uM; DRB2 dsRBD2 triple labelled, [U-100% 13C; U-100% 15N; U-80% 2H], 360 uM; HEPES 20 mM; NaCl 200 mM; DTT 2 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v3.1 - chemical shift assignment, peak picking

TOPSPIN v4.0 - collection, processing

NMR spectrometers:

Bruker AVANCE NEO 600 MHz