BMRB Entry 52606

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52606

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Title:
Assignment and dynamics (hetNOE, T1, T1 rho, T2) of Drosophila Ago2 4-repeats
Deposition date:
2024-09-11
Original release date:
2025-07-01
Authors:
Hipp, Clara; Sattler, Michael
Citation:

Citation: Hipp, Clara; Mussgnug, Selina; Kang, Hyun-Seo; Asami, Sam; Sastre, Judit; Donau, Carsten; Gemmecker, Gerd; Boekhoven, Job; Forstemann, Klaus; Sattler, Michael. "Molecular mechanisms of biomolecular condensate formation in Drosophila melanogaster siRNA biogenesis"  .

Assembly members:

Assembly members:
entity_1, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGHQQGRQGQEGGYQQRPPG QQQGGHQQGRQGQEGGYQQR PSGQQQGGHQQGRQGQEGGY QQRPPGQQQGGHQQGRQGQE GGYQQRPSGQQQ

Data sets:
Data typeCount
13C chemical shifts212
15N chemical shifts88
1H chemical shifts451
T1 relaxation values80
T1rho relaxation values77
T2 relaxation values80
heteronuclear NOE values80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ago2_4repeats1

Entities:

Entity 1, Ago2_4repeats 92 residues - Formula weight is not available

1   GLYGLYHISGLNGLNGLYARGGLNGLYGLN
2   GLUGLYGLYTYRGLNGLNARGPROPROGLY
3   GLNGLNGLNGLYGLYHISGLNGLNGLYARG
4   GLNGLYGLNGLUGLYGLYTYRGLNGLNARG
5   PROSERGLYGLNGLNGLNGLYGLYHISGLN
6   GLNGLYARGGLNGLYGLNGLUGLYGLYTYR
7   GLNGLNARGPROPROGLYGLNGLNGLNGLY
8   GLYHISGLNGLNGLYARGGLNGLYGLNGLU
9   GLYGLYTYRGLNGLNARGPROSERGLYGLN
10   GLNGLN

Samples:

sample_1: Ago2_4repeats, [U-13C; U-15N], 2300 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_2: Ago2_4repeats, [U-13C; U-15N], 1000 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_3: Ago2_4repeats, [U-13C; U-15N], 1000 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_4: Ago2_4repeats, [U-15N], 1000 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_5: Ago2_4repeats, [U-15N], 850 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_5isotropicsample_conditions_1
3D HCCH-TOCSYsample_5isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_5isotropicsample_conditions_1
2D HBCBCGCDHDsample_5isotropicsample_conditions_1
2D HBCBCGCDCEHEsample_5isotropicsample_conditions_1
T1/R1 relaxationsample_4isotropicsample_conditions_1
T2/R2 relaxationsample_4isotropicsample_conditions_1
1H-15N heteronoesample_4isotropicsample_conditions_1
T1rho/R1rho relaxationsample_3isotropicsample_conditions_1

Software:

TOPSPIN v3.5, 3.7 - collection, processing

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AVANCE III 500 MHz
  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 900 MHz
  • Bruker AVANCE III 950 MHz

Related Database Links:

Uniprot Q9VUQ5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks