BMRB Entry 52555

Name: Backbone assignments for TALE Homeodomain Transcription Factor Meis1
Published: 2024-07-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52555

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments for TALE Homeodomain Transcription Factor Meis1

Deposition date:

2024-07-16

Original release date:

2024-07-29

Authors:

Choi, Seo-Ree; Lee, Juyong; Lee, Joon-hwa

Citation:

Citation: Choi, Seo-Ree; Lee, Juyong; Seo, Yeo-Jin; Jin, Ho-Seong; Ahn, Hye-Bin; Go, Youyeon; Kim, Nak-Kyoon; Ryu, Kyoung-Seok; Lee, Joon-Hwa. "Molecular basis of facilitated target search and sequence discrimination of TALE homeodomain transcription factor Meis1" Nat. Commun. 15, 6984-6984 (2024).
PubMed: 39143123

Assembly members:

Assembly members:
entity_1, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RHKKRGIFPKVATNIMRAWL FQHLTHPYPSEEQKKQLAQD TGLTILQVNNWFINARRRIV QPMIDQSNR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	136
15N chemical shifts	77
1H chemical shifts	87

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Meis1-HD	1

Entities:

Entity 1, Meis1-HD 69 residues - Formula weight is not available

1

ARG

HIS

LYS

ARG

GLY

ILE

PHE

PRO

LYS

2

VAL

ALA

THR

ASN

ILE

MET

ARG

ALA

TRP

LEU

3

PHE

GLN

HIS

LEU

THR

HIS

PRO

TYR

PRO

SER

4

GLU

GLN

LYS

GLN

LEU

ALA

GLN

ASP

5

THR

GLY

LEU

THR

ILE

LEU

GLN

VAL

ASN

6

TRP

PHE

ILE

ASN

ALA

ARG

ILE

VAL

7

GLN

PRO

MET

ILE

ASP

GLN

SER

ASN

ARG

Samples:

sample_1: Meis1-HD, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 10 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 110 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

POKY - chemical shift assignment

NMRFAM-SPARKY - chemical shift assignment, peak picking

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE NEO 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks