BMRB Entry 52414

Name: 1H-NMR assignments for the Z0 zinc-finger domain of transcription repressor Bcl11A
Published: 2024-05-20

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PDB ID: 9bv0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52414
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H-NMR assignments for the Z0 zinc-finger domain of transcription repressor Bcl11A

Deposition date:

2024-04-16

Original release date:

2024-05-20

Authors:

Alexandrescu, Andrei

Citation:

Citation: Alexandrescu, Andrei. "Solution structure of the Z0 domain from transcription repressor Bcl11A provides clues about protein-binding zinc finger sequences" To be determined ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 27 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LTCGQCQMNFPLGDILIFIE HKRKQCN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	34
1H chemical shifts	193

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Z0	1
2	ZINC ION	2

Entities:

Entity 1, Z0 27 residues - Formula weight is not available

Corresponds to residues 46-72 of the human BCl11A sequence (UniProt ID: Q9H165)

1

LEU

THR

CYS

GLY

GLN

CYS

GLN

MET

ASN

PHE

2

PRO

LEU

GLY

ASP

ILE

LEU

ILE

PHE

ILE

GLU

3

HIS

LYS

ARG

LYS

GLN

CYS

ASN

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Bcl11A-Z0 40 uM; ZINC ION 600 uM

sample_2: Bcl11A-Z0 40 uM; ZINC ION 600 uM

sample_conditions_1: ionic strength: 0.0006 M; pH: 6.3; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.0006 M; pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_2

Software:

VNMRj - collection

CcpNMR v2.5.2 - chemical shift assignment

X-PLOR NIH v3.8 - structure solution

NMR spectrometers:

Varian INOVA 600 MHz