BMRB Entry 52389

Title:
Phosphorylated Nanog NTD
Deposition date:
2024-04-08
Original release date:
2024-05-09
Authors:
Ferreon, Josephine
Citation:

Citation: Ferreon, Josephine; Ta, Hai Minh; Yun, Hyosuk; Choi, Kyoung-jae; Quan, My Diem; Tsoi, Phoebe; Kim, Choel; Lee, Chul-won; Ferreon, Allan Chris. "Stereospecific NANOG PEST Stabilization by Pin1"  Biochemistry 63, 1067-1074 (2024).
PubMed: 38619104

Assembly members:

Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET302

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ETVXPLPSSMDLLIQDXPDS STXPKG

Data sets:
Data typeCount
13C chemical shifts51
15N chemical shifts21
1H chemical shifts21

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Phosphorylated N-terminal domain1

Entities:

Entity 1, Phosphorylated N-terminal domain 26 residues - Formula weight is not available

1   GLUTHRVALSEPPROLEUPROSERSERMET
2   ASPLEULEUILEGLNASPSEPPROASPSER
3   SERTHRSEPPROLYSGLY

Samples:

sample_1: Phosphorylated Nanog NTD, [U-99% 13C; U-99% 15N], 250 uM; Sodium Phosphate 10 mM

sample_conditions_1: ionic strength: 40 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks