BMRB Entry 52371

Title:
1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium
Deposition date:
2024-03-27
Original release date:
2024-07-19
Authors:
Okmane, Laura; Sandgren, Mats; Stahlberg, Jerry; Nestor, Gustav
Citation:

Citation: Okmane, Laura; Sandgren, Mats; Stahlberg, Jerry; Nestor, Gustav. "1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium"  Biomol. NMR Assignments ., .-. (2024).
PubMed: 38888713

Assembly members:

Assembly members:
entity_1, polymer, 180 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Phanerochaete chrysosporium   Taxonomy ID: 2822231   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Phanerochaete chrysosporium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPICZa

Data sets:
Data typeCount
13C chemical shifts488
15N chemical shifts166
1H chemical shifts555

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PcCel45A1

Entities:

Entity 1, PcCel45A 180 residues - Formula weight is not available

1   ALATHRGLYGLYTYRVALGLNGLNALATHR
2   GLYGLNALASERPHETHRMETTYRSERGLY
3   CYSGLYSERPROALACYSGLYLYSALAALA
4   SERGLYPHETHRALAALAILEASNGLNLEU
5   ALAPHEGLYSERALAPROGLYLEUGLYALA
6   GLYASPALACYSGLYARGCYSPHEALALEU
7   THRGLYASNHISASPPROTYRSERPROASN
8   TYRTHRGLYPROPHEGLYGLNTHRILEVAL
9   VALLYSVALTHRASPLEUCYSPROVALGLN
10   GLYASNGLNGLUPHECYSGLYGLNTHRTHR
11   SERASNPROTHRASNGLNHISGLYMETPRO
12   PHEHISPHEASPILECYSGLUASPTHRGLY
13   GLYSERALALYSPHEPHEPROSERGLYHIS
14   GLYALALEUTHRGLYTHRPHETHRGLUVAL
15   SERCYSSERGLNTRPSERGLYSERASPGLY
16   GLYGLNLEUTRPASNGLYALACYSLEUSER
17   GLYGLUTHRALAPROASNTRPPROSERTHR
18   ALACYSGLYASNLYSGLYTHRALAPROSER

Samples:

sample_1: PcCel45A, [U-70% 13C; U-95% 15N], 450 uM; D2O, [U-99% 2H], 10%; potassium phosphate 50 mM; sodium azide 0.03%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.1.4 - collection, processing

CcpNMR - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE III 600 MHz MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks