BMRB Entry 52362

Name: Backbone assignment of Hrp48 RRM12 bound to 10mer RNA
Published: 2024-12-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52362

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of Hrp48 RRM12 bound to 10mer RNA

Deposition date:

2024-03-25

Original release date:

2024-12-26

Authors:

Lomoschitz, Andrea; Meyer, Julia; Schweimer, Kristian; Hennig, Janosch

Citation:

Citation: Lomoschitz, Andrea; Meyer, Julia; Guitart, Tanit; Krepl, Miroslav; Lapouge, Karine; Hayn, Clara; Schweimer, Kristian; Simon, Bernd; Sponer, Jiri; Gebauer, Fatima; Hennig, Janosch. "The Drosophila RNA binding protein Hrp48 binds a specific RNA sequence of the msl-2 mRNA 3' UTR to regulate translation" Biophys. Chem. 316, 107346-107346 (2025).
PubMed: 39504588

Assembly members:

Assembly members:
entity_1, polymer, 175 residues, Formula weight is not available
entity_2, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMEEDERGKLFVGGLSWET TQENLSRYFCRFGDIIDCVV MKNNESGRSRGFGFVTFADP TNVNHVLQNGPHTLDGRTID PKPCNPRTLQKPKKGGGYKV FLGGLPSNVTETDLRTFFNR YGKVTEVVIMYDQEKKKSRG FGFLSFEEESSVEHVTNERY INLNGKQVEIKKAEP
entity_2: UAGGAUUAAG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	306
15N chemical shifts	160
1H chemical shifts	160

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hrp48 RRM12	1
2	10mer RNA	2

Entities:

Entity 1, Hrp48 RRM12 175 residues - Formula weight is not available

N-terminal GA from expression tag

1

GLY

ALA

MET

GLU

ASP

GLU

ARG

GLY

LYS

2

LEU

PHE

VAL

GLY

LEU

SER

TRP

GLU

THR

3

THR

GLN

GLU

ASN

LEU

SER

ARG

TYR

PHE

CYS

4

ARG

PHE

GLY

ASP

ILE

ASP

CYS

VAL

5

MET

LYS

ASN

GLU

SER

GLY

ARG

SER

ARG

6

GLY

PHE

GLY

PHE

VAL

THR

PHE

ALA

ASP

PRO

7

THR

ASN

VAL

ASN

HIS

VAL

LEU

GLN

ASN

GLY

8

PRO

HIS

THR

LEU

ASP

GLY

ARG

THR

ILE

ASP

9

PRO

LYS

PRO

CYS

ASN

PRO

ARG

THR

LEU

GLN

10

LYS

PRO

LYS

GLY

TYR

LYS

VAL

11

PHE

LEU

GLY

LEU

PRO

SER

ASN

VAL

THR

12

GLU

THR

ASP

LEU

ARG

THR

PHE

ASN

ARG

13

TYR

GLY

LYS

VAL

THR

GLU

VAL

ILE

MET

14

TYR

ASP

GLN

GLU

LYS

SER

ARG

GLY

15

PHE

GLY

PHE

LEU

SER

PHE

GLU

SER

16

SER

VAL

GLU

HIS

VAL

THR

ASN

GLU

ARG

TYR

17

ILE

ASN

LEU

ASN

GLY

LYS

GLN

VAL

GLU

ILE

18

LYS

ALA

GLU

PRO

Entity 2, 10mer RNA 10 residues - Formula weight is not available

1

U

A

G

A

U

A

G

Samples:

sample_1: Hrp48 RRM12, [U-95% 13C; U-95% 15N], 205 uM; 10mer RNA 307.5 uM; D2O, [U-2H], 5%; MES 20 mM; sodium chloride 200 mM

sample_conditions_1: ionic strength: 0.4 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks