Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52336

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Backbone 1H and 15N Chemical Shift Assignments for phosHP1amutant
Published: 2026-04-14

Title:

Backbone 1H and 15N Chemical Shift Assignments for phosHP1amutant

Deposition date:

2024-02-20

Original release date:

2026-04-14

Authors:

Furukawa, Ayako; Nishimura, Yoshifumi

Citation:

Citation: Furukawa, Ayako; Yonezawa, Kento; Negami, Tatsuki; Yoshimura, Yuriko; Hayashi, Aki; Nakayama, Jun-Ichi; Adachi, Naruhiko; Senda, Toshiya; Shimizu, Kentaro; Terada, Tohru; Shimizu, Nobutaka; Nishimura, Yoshifumi. "A dynamic structural unit of phase-separated heterochromatin protein 1a as revealed by integrative structural analyses" Nucleic Acids Res. 53, gkaf154-gkaf154 (2025).
PubMed: 40138713

Assembly members:

Assembly members:
entity_1, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGKKTKRTADSSSSEDEEEY VVEKVLDRRVVKGQVEYLLK WKGFSEEHNTWEPEKNLDCP ELISEFMKKYKKMKEGENNK PREKSESNKRKSNFSNSADD IKSKKKREQSNDIARGFERG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	104
1H chemical shifts	104

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pHP1aNCDhinge	1

Entities:

Entity 1, pHP1aNCDhinge 120 residues - Formula weight is not available

1	MET	GLY	LYS	LYS	THR	LYS	ARG	THR	ALA	ASP
2	SER	SER	SER	SER	GLU	ASP	GLU	GLU	GLU	TYR
3	VAL	VAL	GLU	LYS	VAL	LEU	ASP	ARG	ARG	VAL
4	VAL	LYS	GLY	GLN	VAL	GLU	TYR	LEU	LEU	LYS
5	TRP	LYS	GLY	PHE	SER	GLU	GLU	HIS	ASN	THR
6	TRP	GLU	PRO	GLU	LYS	ASN	LEU	ASP	CYS	PRO
7	GLU	LEU	ILE	SER	GLU	PHE	MET	LYS	LYS	TYR
8	LYS	LYS	MET	LYS	GLU	GLY	GLU	ASN	ASN	LYS
9	PRO	ARG	GLU	LYS	SER	GLU	SER	ASN	LYS	ARG
10	LYS	SER	ASN	PHE	SER	ASN	SER	ALA	ASP	ASP
11	ILE	LYS	SER	LYS	LYS	LYS	ARG	GLU	GLN	SER
12	ASN	ASP	ILE	ALA	ARG	GLY	PHE	GLU	ARG	GLY

Samples:

sample_1: pHP1aNCDhinge, [U-100% 13C; U-100% 15N], 400 uM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 296.7 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1

Software:

MAGRO - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	GLY	LYS	LYS	THR	LYS	ARG	THR	ALA	ASP
2	SER	SER	SER	SER	GLU	ASP	GLU	GLU	GLU	TYR
3	VAL	VAL	GLU	LYS	VAL	LEU	ASP	ARG	ARG	VAL
4	VAL	LYS	GLY	GLN	VAL	GLU	TYR	LEU	LEU	LYS
5	TRP	LYS	GLY	PHE	SER	GLU	GLU	HIS	ASN	THR
6	TRP	GLU	PRO	GLU	LYS	ASN	LEU	ASP	CYS	PRO
7	GLU	LEU	ILE	SER	GLU	PHE	MET	LYS	LYS	TYR
8	LYS	LYS	MET	LYS	GLU	GLY	GLU	ASN	ASN	LYS
9	PRO	ARG	GLU	LYS	SER	GLU	SER	ASN	LYS	ARG
10	LYS	SER	ASN	PHE	SER	ASN	SER	ALA	ASP	ASP
11	ILE	LYS	SER	LYS	LYS	LYS	ARG	GLU	GLN	SER
12	ASN	ASP	ILE	ALA	ARG	GLY	PHE	GLU	ARG	GLY

1	MET	GLY	LYS	LYS	THR	LYS	ARG	THR	ALA	ASP
2	SER	SER	SER	SER	GLU	ASP	GLU	GLU	GLU	TYR
3	VAL	VAL	GLU	LYS	VAL	LEU	ASP	ARG	ARG	VAL
4	VAL	LYS	GLY	GLN	VAL	GLU	TYR	LEU	LEU	LYS
5	TRP	LYS	GLY	PHE	SER	GLU	GLU	HIS	ASN	THR
6	TRP	GLU	PRO	GLU	LYS	ASN	LEU	ASP	CYS	PRO
7	GLU	LEU	ILE	SER	GLU	PHE	MET	LYS	LYS	TYR
8	LYS	LYS	MET	LYS	GLU	GLY	GLU	ASN	ASN	LYS
9	PRO	ARG	GLU	LYS	SER	GLU	SER	ASN	LYS	ARG
10	LYS	SER	ASN	PHE	SER	ASN	SER	ALA	ASP	ASP
11	ILE	LYS	SER	LYS	LYS	LYS	ARG	GLU	GLN	SER
12	ASN	ASP	ILE	ALA	ARG	GLY	PHE	GLU	ARG	GLY

BMRB Entry 52336