BMRB Entry 52309

Name: Backbone assignment of a Tau fragment (272-441) from Tau 4R isoforms
Published: 2025-03-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52309

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of a Tau fragment (272-441) from Tau 4R isoforms

Deposition date:

2024-02-06

Original release date:

2025-03-25

Authors:

El Hajjar, Lea; Boll, Emmanuelle; Cantrelle, Francois-Xavier; Smet-Nocca, Caroline

Citation:

Citation: El Hajjar, Lea; Boll, Emmanuelle; Cantrelle, Francois-Xavier; Bridot, Clarisse; Landrieu, Isabelle; Smet-Nocca, Caroline. "Effect of PHF-1 hyperphosphorylation on the seeding activity of C-terminal Tau fragments" Sci. Rep. 15, 9975-9975 (2025).
PubMed: 40121258

Assembly members:

Assembly members:
entity_1, polymer, 170 residues, 17947.3892 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGKVQIINKKLDLSNVQSKC GSKDNIKHVPGGGSVQIVYK PVDLSKVTSKCGSLGNIHHK PGGGQVEVKSEKLDFKDRVQ SKIGSLDNITHVPGGGNKKI ETHKLTFRENAKAKTDHGAE IVYKSPVVSGDTSPRHLSNV SSTGSIDMVDSPQLATLADE VSASLAKQGL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	954
15N chemical shifts	328
1H chemical shifts	640

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	R2Ct-WT	1

Entities:

Entity 1, R2Ct-WT 170 residues - 17947.3892 Da.

1	GLY	GLY	LYS	VAL	GLN	ILE	ILE	ASN	LYS	LYS
2	LEU	ASP	LEU	SER	ASN	VAL	GLN	SER	LYS	CYS
3	GLY	SER	LYS	ASP	ASN	ILE	LYS	HIS	VAL	PRO
4	GLY	GLY	GLY	SER	VAL	GLN	ILE	VAL	TYR	LYS
5	PRO	VAL	ASP	LEU	SER	LYS	VAL	THR	SER	LYS
6	CYS	GLY	SER	LEU	GLY	ASN	ILE	HIS	HIS	LYS
7	PRO	GLY	GLY	GLY	GLN	VAL	GLU	VAL	LYS	SER
8	GLU	LYS	LEU	ASP	PHE	LYS	ASP	ARG	VAL	GLN
9	SER	LYS	ILE	GLY	SER	LEU	ASP	ASN	ILE	THR
10	HIS	VAL	PRO	GLY	GLY	GLY	ASN	LYS	LYS	ILE
11	GLU	THR	HIS	LYS	LEU	THR	PHE	ARG	GLU	ASN
12	ALA	LYS	ALA	LYS	THR	ASP	HIS	GLY	ALA	GLU
13	ILE	VAL	TYR	LYS	SER	PRO	VAL	VAL	SER	GLY
14	ASP	THR	SER	PRO	ARG	HIS	LEU	SER	ASN	VAL
15	SER	SER	THR	GLY	SER	ILE	ASP	MET	VAL	ASP
16	SER	PRO	GLN	LEU	ALA	THR	LEU	ALA	ASP	GLU
17	VAL	SER	ALA	SER	LEU	ALA	LYS	GLN	GLY	LEU

Samples:

sample_1: R2Ct-WT, [U-100% 13C; U-100% 15N], 0.300 mM; NaPi 25 mM; NaCl 50 mM; EDTA 2.5 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.092 M; pH: 6.600; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis v2.5 - peak picking

TOPSPIN v4.3.1 - acquisition

qMDD v2.6 - processing

NMR spectrometers:

Bruker Avance 900 MHz

1	GLY	GLY	LYS	VAL	GLN	ILE	ILE	ASN	LYS	LYS
2	LEU	ASP	LEU	SER	ASN	VAL	GLN	SER	LYS	CYS
3	GLY	SER	LYS	ASP	ASN	ILE	LYS	HIS	VAL	PRO
4	GLY	GLY	GLY	SER	VAL	GLN	ILE	VAL	TYR	LYS
5	PRO	VAL	ASP	LEU	SER	LYS	VAL	THR	SER	LYS
6	CYS	GLY	SER	LEU	GLY	ASN	ILE	HIS	HIS	LYS
7	PRO	GLY	GLY	GLY	GLN	VAL	GLU	VAL	LYS	SER
8	GLU	LYS	LEU	ASP	PHE	LYS	ASP	ARG	VAL	GLN
9	SER	LYS	ILE	GLY	SER	LEU	ASP	ASN	ILE	THR
10	HIS	VAL	PRO	GLY	GLY	GLY	ASN	LYS	LYS	ILE
11	GLU	THR	HIS	LYS	LEU	THR	PHE	ARG	GLU	ASN
12	ALA	LYS	ALA	LYS	THR	ASP	HIS	GLY	ALA	GLU
13	ILE	VAL	TYR	LYS	SER	PRO	VAL	VAL	SER	GLY
14	ASP	THR	SER	PRO	ARG	HIS	LEU	SER	ASN	VAL
15	SER	SER	THR	GLY	SER	ILE	ASP	MET	VAL	ASP
16	SER	PRO	GLN	LEU	ALA	THR	LEU	ALA	ASP	GLU
17	VAL	SER	ALA	SER	LEU	ALA	LYS	GLN	GLY	LEU

1	GLY	GLY	LYS	VAL	GLN	ILE	ILE	ASN	LYS	LYS
2	LEU	ASP	LEU	SER	ASN	VAL	GLN	SER	LYS	CYS
3	GLY	SER	LYS	ASP	ASN	ILE	LYS	HIS	VAL	PRO
4	GLY	GLY	GLY	SER	VAL	GLN	ILE	VAL	TYR	LYS
5	PRO	VAL	ASP	LEU	SER	LYS	VAL	THR	SER	LYS
6	CYS	GLY	SER	LEU	GLY	ASN	ILE	HIS	HIS	LYS
7	PRO	GLY	GLY	GLY	GLN	VAL	GLU	VAL	LYS	SER
8	GLU	LYS	LEU	ASP	PHE	LYS	ASP	ARG	VAL	GLN
9	SER	LYS	ILE	GLY	SER	LEU	ASP	ASN	ILE	THR
10	HIS	VAL	PRO	GLY	GLY	GLY	ASN	LYS	LYS	ILE
11	GLU	THR	HIS	LYS	LEU	THR	PHE	ARG	GLU	ASN
12	ALA	LYS	ALA	LYS	THR	ASP	HIS	GLY	ALA	GLU
13	ILE	VAL	TYR	LYS	SER	PRO	VAL	VAL	SER	GLY
14	ASP	THR	SER	PRO	ARG	HIS	LEU	SER	ASN	VAL
15	SER	SER	THR	GLY	SER	ILE	ASP	MET	VAL	ASP
16	SER	PRO	GLN	LEU	ALA	THR	LEU	ALA	ASP	GLU
17	VAL	SER	ALA	SER	LEU	ALA	LYS	GLN	GLY	LEU