BMRB Entry 52291

Name: 1H,15N and 13C backbone chemical shift assignment of WDR5
Published: 2025-12-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52291

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H,15N and 13C backbone chemical shift assignment of WDR5

Deposition date:

2024-01-29

Original release date:

2025-12-20

Authors:

Wu, Bin; Wang, Duo; Li, Yanjing; Chen, Yong

Citation:

Citation: Wang, Duo; Li, Yanjing; Chen, Yong. "WDR5 Reshapes Nanog Prion-like Structure to Sustain Pluripotency in Embryonic Stem Cells" .

Assembly members:

Assembly members:
entity_1, polymer, 312 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ATQSKPTPVKPNYALKFTLA GHTKAVSSVKFSPNGEWLAS SSADKLIKIWGAYDGKFEKT ISGHKLGISDVAWSSDSNLL VSASDDKTLKIWDVSSGKCL KTLKGHSNYVFCCNFNPQSN LIVSGSFDESVRIWDVKTGK CLKTLPAHSDPVSAVHFNRD GSLIVSSSYDGLCRIWDTAS GQCLKTLIDDDNPPVSFVKF SPNGKYILAATLDNTLKLWD YSKGKCLKTYTGHKNEKYCI FANFSVTGGKWIVSGSEDNL VYIWNLQTKEIVQKLQGHTD VVISTACHPTENIIASAALE NDKTIKLWKSDC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	828
15N chemical shifts	259
1H chemical shifts	259

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WDR5	1

Entities:

Entity 1, WDR5 312 residues - Formula weight is not available

1

ALA

THR

GLN

SER

LYS

PRO

THR

PRO

VAL

LYS

2

PRO

ASN

TYR

ALA

LEU

LYS

PHE

THR

LEU

ALA

3

GLY

HIS

THR

LYS

ALA

VAL

SER

VAL

LYS

4

PHE

SER

PRO

ASN

GLY

GLU

TRP

LEU

ALA

SER

5

SER

ALA

ASP

LYS

LEU

ILE

LYS

ILE

TRP

6

GLY

ALA

TYR

ASP

GLY

LYS

PHE

GLU

LYS

THR

7

ILE

SER

GLY

HIS

LYS

LEU

GLY

ILE

SER

ASP

8

VAL

ALA

TRP

SER

ASP

SER

ASN

LEU

9

VAL

SER

ALA

SER

ASP

LYS

THR

LEU

LYS

10

ILE

TRP

ASP

VAL

SER

GLY

LYS

CYS

LEU

11

LYS

THR

LEU

LYS

GLY

HIS

SER

ASN

TYR

VAL

12

PHE

CYS

ASN

PHE

ASN

PRO

GLN

SER

ASN

13

LEU

ILE

VAL

SER

GLY

SER

PHE

ASP

GLU

SER

14

VAL

ARG

ILE

TRP

ASP

VAL

LYS

THR

GLY

LYS

15

CYS

LEU

LYS

THR

LEU

PRO

ALA

HIS

SER

ASP

16

PRO

VAL

SER

ALA

VAL

HIS

PHE

ASN

ARG

ASP

17

GLY

SER

LEU

ILE

VAL

SER

TYR

ASP

18

GLY

LEU

CYS

ARG

ILE

TRP

ASP

THR

ALA

SER

19

GLY

GLN

CYS

LEU

LYS

THR

LEU

ILE

ASP

20

ASP

ASN

PRO

VAL

SER

PHE

VAL

LYS

PHE

21

SER

PRO

ASN

GLY

LYS

TYR

ILE

LEU

ALA

22

THR

LEU

ASP

ASN

THR

LEU

LYS

LEU

TRP

ASP

23

TYR

SER

LYS

GLY

LYS

CYS

LEU

LYS

THR

TYR

24

THR

GLY

HIS

LYS

ASN

GLU

LYS

TYR

CYS

ILE

25

PHE

ALA

ASN

PHE

SER

VAL

THR

GLY

LYS

26

TRP

ILE

VAL

SER

GLY

SER

GLU

ASP

ASN

LEU

27

VAL

TYR

ILE

TRP

ASN

LEU

GLN

THR

LYS

GLU

28

ILE

VAL

GLN

LYS

LEU

GLN

GLY

HIS

THR

ASP

29

VAL

ILE

SER

THR

ALA

CYS

HIS

PRO

THR

30

GLU

ASN

ILE

ALA

SER

ALA

LEU

GLU

31

ASN

ASP

LYS

THR

ILE

LYS

LEU

TRP

LYS

SER

32

ASP

CYS

Samples:

sample_1: WDR5, [U-13C; U-15N; U-2H], 0.5 mM; HEPS 25 mM; NaCl 300 mM; DTT 4 mM; NaN3 0.03%

sample_conditions_1: ionic strength: 0.3 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

PINE - chemical shift assignment

NMRFAM-SPARKY - data analysis

NMR spectrometers:

Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks